Catalog Ready-to-Use Lyophilisates - Reverse Transcription Master LyophilisatesReady-to-Use Lyophilisates - Reverse Transcription Master Lyophilisates
 
 
Real-Time Master Lyophilisates   |   Direct PCR and Multiplex PCR Lyophilisates   |   Taq & Hot Start Master Lyophilisates   |   Reverse Transcription Master Lyophilisates   |   Custom-Specific Lyophilizates
 
PCR Lyophilisates
Lyophilisates Preloaded and Stable at Room Temperature

Ready-to-Use Lyophilisates are delivered in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisates combine highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations. Each vial contains enzyme, dNTPs and reaction buffer required for a 20 μl PCR assay. Simply fill up the vials with a premix of RNA template and primers and run the reverse transcripton in a thermocycler as usual.



Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, enzyme, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template RNA, filling up with water and starting the cycler!
 

One-Step RT-PCR Master Lyophilisate
Lyophilized One-Step RT-PCR Master Mix
   
Product Cat. No. Amount Price (EUR) Buy / Note  
preloaded 8-tube strips PCR-159S-8TS 12 strips
(96 reactions)
340,00    Add this product to your notepad  
preloaded 8-tube strips PCR-159L-8TS 60 strips
(480 reactions)
1.360,00    Add this product to your notepad
preloaded 96-well plates
flat top / without skirt
PCR-159S-FTP 2 plates
(192 reactions)
510,00    Add this product to your notepad
preloaded 96-well plates
flat top / without skirt
PCR-159L-FTP 10 plates
(960 reactions)
2.040,00    Add this product to your notepad
preloaded 96-well plates
half skirt
PCR-159S-HSP 2 plates
(192 reactions)
510,00    Add this product to your notepad
preloaded 96-well plates
half skirt
PCR-159L-HSP 10 plates
(960 reactions)
2.040,00    Add this product to your notepad

Quality guaranteed for 12 months when stored below 25C

One-Step RT-PCR Master Lyophilisate
PCR-grade water
One-Step RT-PCR Master Lyophilisate is designed for performing maximum sensitive and specific RT-PCRs convenient in single tubes. The enzyme mix is based on a genetically engineered RT polymerase with enhanced thermal stability resulting in an increased specificity, higher cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The lyophilisate contains all reagents required for RT-PCR (except template and primer) in one tube to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mix, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
 
RT Master Lyophilisate
Lyophilized Master Mix for Reverse Transcription
   
Product Cat. No. Amount Price (EUR) Buy / Note  
preloaded 8-tube strips PCR-158S-8TS 12 strips
(96 reactions)
280,00    Add this product to your notepad  
preloaded 8-tube strips PCR-158L-8TS 60 strips
(480 reactions)
1.120,00    Add this product to your notepad
preloaded 96-well plates
flat top / without skirt
PCR-158S-FTP 2 plates
(192 reactions)
420,00    Add this product to your notepad
preloaded 96-well plates
flat top / without skirt
PCR-158L-FTP 10 plates
(960 reactions)
1.680,00    Add this product to your notepad
preloaded 96-well plates
half skirt
PCR-158S-HSP 2 plates
(192 reactions)
420,00    Add this product to your notepad
preloaded 96-well plates
half skirt
PCR-158L-HSP 10 plates
(960 reactions)
1.680,00    Add this product to your notepad

Quality guaranteed for 12 months when stored below 25C

RT Master Lyophilisate
PCR-grade water
RT Master Lyophilisate contains a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Each vial contains all components (except primers and template) required for a 20 l reverse transcription assay. To perform the assay, only fill up the vials with a mix of primers and RNA template.