Click chemistry is a chemical philosophy introduced by K. Barry Sharpless in 2001 and describes chemical reactions that generate substances quickly, reliably and in quantitative yield by joining small building blocks under mild conditions. This is inspired by the fact that chemical reactions in nature also generate substances by joining small modular units.

One of the most popular reactions within the click chemistry philosophy is the so called azide-alkyne Huisgen cycloaddition, using a Copper (Cu) catalyst at room temperature.

These nucleoside triphosphates are used to generate DNA containing reactive groups (triple bonds) by PCR. These groups enable the attachment of reporter groups such as biotin or fluorescent dyes by click chemistry. This allows the synthesis of highly-modified DNA strands carrying multiple labels in a density that is not achieved by classic labeling techniques.

Please see our Click Chemistry catalog section for more click chemistry related products.

Please contact nucleotides@jenabioscience.com with questions or inquiries.