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Reverse Transcription - One-Step RT-PCROne-Step RT-PCR
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Jena Bioscience one-step RT-PCR kits contain all reagents required for RT-PCR (except template and primer) in one box to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mix and the optimized complete reaction buffer containing ultrapure dNTPs ensure superior amplification results. This offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA and minimizes the risk of contaminations.
| SCRIPT One-Step RT-PCR Kit One-Step RT-PCR Kit for highly sensitive and specific amplification |
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| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| XS pack | PCR-509XS | 500 µl 2 x conc. |
95,00 | 
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| S pack | PCR-509S | 2,5 ml 2 x conc. |
380,00 |
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| L pack | PCR-509L | 12,5 ml 2 x conc. |
1.520,00 |
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|
Store at -20°C, avoid frequent thawing and freezing
SCRIPT RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start Polymerase, RNase Inhibitor)
2x SCRIPT RT-PCR Reaction Mix
RNase-free Water
SCRIPT RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start Polymerase, RNase Inhibitor)
2x SCRIPT RT-PCR Reaction Mix
RNase-free Water
SCRIPT One-Step RT-PCR Kit is designed for performing highly sensitive and specific RT-PCR convenient in single tubes. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
| SCRIPT High Fidelity One-Step RT-PCR Kit One-Step RT-PCR Kit for highly precise and fast amplification |
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| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| XS pack | PCR-510XS | 500 µl 2 x conc. |
108,00 |
|
|
| S pack | PCR-510S | 2,5 ml 2 x conc. |
432,00 |
|
|
| L pack | PCR-510L | 12,5 ml 2 x conc. |
1.728,00 |
|
|
Store at -20°C, avoid frequent thawing and freezing
SCRIPT High Fidelity RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start High Fidelity Polymerase, RNase Inhibitor)
2x SCRIPT High Fidelity RT-PCR Reaction Mix
RNase-free Water
SCRIPT High Fidelity RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start High Fidelity Polymerase, RNase Inhibitor)
2x SCRIPT High Fidelity RT-PCR Reaction Mix
RNase-free Water
SCRIPT High Fidelity One-Step RT-PCR Kit is the ideal choice for applications where highly sensitive reverse transcription and high fidelity PCR at high amplification speed in single tubes are required. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. Additionally, a genetically engineered proof-reading enzyme is implemented as polymerase. It provides a 50-fold higher accuracy and an increased processivity compared to Taq, resulting in 2-fold shorter elongation times.