. One-Step RT-PCR, Reverse Transcription, Molecular Biology, Products & Ordering, Jena Bioscience
 
 
Catalog Reverse Transcription - One-Step RT-PCRReverse Transcription - One-Step RT-PCR
 
 
One-Step RT-qPCR   |   One-Step RT-PCR   |   Two-Step RT-PCR   |   Reverse Transcriptases   |   Supplements
 

One-Step RT-PCR


SCRIPT One-Step RT-qPCR Master Mixes allow quantitative mRNA detection by reverse transcription and real-time PCR. Reactions are carried out sequentially in a single tube, thus minimizing pipetting steps and contamination risk. Targets from <1 pg mRNA or 10 pg total RNA are detectable. All mixes are supplied as 2x concentrated solution containing all reagents required for RT-qPCR (except template and primers). Kits are available in combination with Dual Labeled Fluorescent Probes or EvaGreenÖ fluorescent DNA stain.

 

SCRIPT One-Step RT-PCR Kit
One-Step RT-PCR Kit for highly sensitive and specific amplification
   
Product Cat. No. Amount Price (EUR) Buy / Note  
XS pack PCR-509XS 20 reactions x 50 Ál 95,00    Add this product to your notepad  
S pack PCR-509S 100 reactions x 50 Ál 380,00    Add this product to your notepad
L pack PCR-509L 500 reactions x 50 Ál 1.520,00    Add this product to your notepad

Store at -20░C, avoid frequent thawing and freezing

SCRIPT RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start Polymerase, RNase Inhibitor)
2x SCRIPT RT-PCR Reaction Mix
RNase-free Water
SCRIPT One-Step RT-PCR Kit is designed for performing highly sensitive and specific RT-PCR convenient in single tubes. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
 
SCRIPT High Fidelity One-Step RT-PCR Kit
One-Step RT-PCR Kit for highly precise and fast amplification
   
Product Cat. No. Amount Price (EUR) Buy / Note  
XS pack PCR-510XS 20 reactions x 50 Ál 108,00    Add this product to your notepad  
S pack PCR-510S 100 reactions x 50 Ál 432,00    Add this product to your notepad
L pack PCR-510L 500 reactions x 50 Ál 1.728,00    Add this product to your notepad

Store at -20░C, avoid frequent thawing and freezing

SCRIPT High Fidelity RT-PCR Enzyme Mix
(incl. SCRIPT RTase, Hot Start High Fidelity Polymerase, RNase Inhibitor)
2x SCRIPT High Fidelity RT-PCR Reaction Mix
RNase-free Water
SCRIPT High Fidelity One-Step RT-PCR Kit is the ideal choice for applications where highly sensitive reverse transcription and high fidelity PCR at high amplification speed in single tubes are required. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. Additionally, a genetically engineered proof-reading enzyme is implemented as polymerase. It provides a 50-fold higher accuracy and an increased processivity compared to Taq, resulting in 2-fold shorter elongation times.