Catalog JBScreen Family - JBScreen Nuc-ProJBScreen Family - JBScreen Nuc-Pro
 
 
JBScreen Classic   |   JBScreen Basic   |   JBScreen Membrane   |   JBScreen Kinase   |   JBScreen Nuc-Pro   |   JBScreen PEG/Salt   |   JBScreen Pentaerythritol   |   JBScreen PACT++   |   JBScreen JCSG++   |   Pi-minimal Screen   |   Pi-PEG Screen   |   JBScreen Wizard
 
JBScreen bulk & HTS
JBScreen Nuc-Pro is designed to screen for preliminary crystallization conditions of nucleic acids and protein-nucleic acid complexes.

The highly effective sparse matrix screen is based upon extensive screening of the PDB [1], with focus on entries by structural genomic initiatives, the BMCD [2] and other protocols [3-5]. Reported crystallization conditions for various RNAs, DNAs as well as protein-nucleic acid complexes were compiled and analyzed for rate of recurrence.
The 96 conditions selected cover a variety of polymers, mono- and divalent metal ions, organics, alcohols and buffers of a pH range from 4.0 to 8.5. The organization of the reagents into individual kits is based upon the main precipitant, i.e. various molecular weight PEGs, Salts, alcohols (MPD and 2-Propanol).

JBScreen Nuc-Pro is available as 4 individual kits containing 24 reagents each in 10 ml bulk format or in a pre-filled 96 deep well block.

The ready-to-use reagents are tested for DNase contamination using our DNase Detection Kit.
 


Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
 
 
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
JBScreen Nuc-Pro 1 CS-181 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Nuc-Pro 2 CS-182 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Nuc-Pro 3 CS-183 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Nuc-Pro 4 CS-184 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Nuc-Pro 1 - 4 CS-185 4 Kits 450,00    Add this product to your notepad
JBScreen Nuc-Pro HTS CS-209L 96 solutions
(1,7 ml each)
225,00    Add this product to your notepad      

References and Recommended Reading:

[1] Berman et al. (2000) The Protein Data Bank. Nucleic Acids Research 28:235.
[2] Gilliland et al. (1994) The Biological Macromolecule Crystallization Database, Version 3.0: New Features, Data, and the NASA Archive for Protein Crystal Growth Data. Acta Cryst. D50:408.
[3] Doudna et al. (1993) Crystallization of ribozymes and small RNA motifs by a sparse matrix approach. Proc. Natl. Sci. USA 90:7829.
[4] Scott et al. (1995) Rapid Crystallization of Chemically Synthesized Hammerhead RNAs using a Double Screening Procedure. J. Mol. Biol. 250:327.
[5] Ke et al. (2004) Crystallization of RNA and RNA-protein complexes. Methods 34:408.
 


Selected Literature Citations of JBScreen Nuc-Pro

  • Tishchenko et al. (2013) Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036. Acta Cryst. F 69:61.
 


Please contact xtals@jenabioscience.com with questions or inquiries.