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Crystallization Screens - JBScreen Nuc-Pro |
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JBScreen Nuc-Pro is designed to screen for preliminary crystallization conditions of nucleic acids and protein-nucleic acid complexes.
The highly effective sparse matrix screen is based upon extensive screening of the PDB [1], with focus on entries by structural genomic initiatives, the BMCD [2] and other protocols [3-5]. Reported crystallization conditions for various RNAs, DNAs as well as protein-nucleic acid complexes were compiled and analyzed for rate of recurrence.
The 96 conditions selected cover a variety of polymers, mono- and divalent metal ions, organics, alcohols and buffers of a pH range from 4.0 to 8.5. The organization of the reagents into individual kits is based upon the main precipitant, i.e. various molecular weight PEGs, Salts, alcohols (MPD and 2-Propanol).
JBScreen Nuc-Pro is available as 4 individual kits containing 24 reagents each in 10 ml bulk format or in a pre-filled 96 deep well block.
The ready-to-use reagents are tested for DNase contamination using our DNase Detection Kit.
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Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
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 Description how the JBScreen buffers are prepared |
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Product |
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Cat. No. |
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Amount |
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Price (EUR) |
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Buy |
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Downloads |
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JBScreen Nuc-Pro 1 |
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CS-181 |
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1 Kit |
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150,00 |
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JBScreen Nuc-Pro 2 |
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CS-182 |
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1 Kit |
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150,00 |
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JBScreen Nuc-Pro 3 |
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CS-183 |
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1 Kit |
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150,00 |
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JBScreen Nuc-Pro 4 |
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CS-184 |
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1 Kit |
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150,00 |
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JBScreen Nuc-Pro 1 - 4 |
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CS-185 |
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4 Kits |
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450,00 |
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JBScreen Nuc-Pro HTS S
(1.0 ml per well) |
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CS-209S |
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1 Block |
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140,00 |
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JBScreen Nuc-Pro HTS L
(1.7 ml per well) |
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CS-209L |
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1 Block |
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225,00 |
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References and Recommended Reading:
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[1] Berman et al. (2000) The Protein Data Bank. Nucleic Acids Research 28:235.
[2] Gilliland et al. (1994) The Biological Macromolecule Crystallization Database, Version 3.0: New Features, Data, and the NASA Archive for Protein Crystal Growth Data. Acta Cryst. D50:408.
[3] Doudna et al. (1993) Crystallization of ribozymes and small RNA motifs by a sparse matrix approach. Proc. Natl. Sci. USA 90:7829.
[4] Scott et al. (1995) Rapid Crystallization of Chemically Synthesized Hammerhead RNAs using a Double Screening Procedure. J. Mol. Biol. 250:327.
[5] Ke et al. (2004) Crystallization of RNA and RNA-protein complexes. Methods 34:408.
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Please contact xtals@jenabioscience.com with questions or inquiries.
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