Recognition Sequence:


Source: Acetobacter pasteurianus

Buffer supplied: 10x Reaction Buffer B1.

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 μg/ml BSA and 50% [v/v] glycerol.
Store at -20°C.

Reaction conditions: 10 mM Tris-HCl (pH 7.9), 10 mM MgCl₂, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.

Heat inactivation: No.

Ligation and recutting: After 100-fold overdigestion with ApaL I,
> 99 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation:
No Inhibition: dcm, dam
Inhibition (Blocked by overlapping): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (4 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.