 | Source: Acetobacter pasteurianus
Buffer supplied: 10x B1 (incl. 10x BSA).
Substrate for unit definition: λ DNA (4 sites).
Reaction conditions: 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Ligation and recutting: After 100-fold overdigestion with ApaL I, > 99 % of the
DNA fragments can be ligated and recut with this
enzyme.
Heat inactivation: No. | |
