Application
Heavy atom derivatization of biological macromolecules for isomorphous and/or anomalous phasing methods.

Kit Contents
6 pre-weighted solid aliquots of hexatantalum tetradecabromide at 1 mg.

The Tantalum Bromide Cluster, manufactured by Proteros biostructures GmbH, is utilized for the preparation of heavy-atom derivatives for structure determination of biological macromolecules by X-ray analysis.

The electron-rich compound can be employed in single and multiple isomorphous replacement (SIR and MIR) experiments in order to induce measurable changes of the diffraction intensities required for phase calculation.
Furthermore, the Tantalum Bromide Cluster contains two strong anomalous scatterers suitable for multiple anomalous diffraction (MAD) experiments.
Tantalum Bromide Clusters have been successfully employed in several structural studies because of their high electron-density, solubility in aqueous solutions and stability over a wide pH range [1-4].

• Szczepanowski et al. (2005) Crystal structure of a fragment of mouse ubiquitin-activating enzyme. J. Biol. Chem. 280:22006.
• Gomis-Rüth et al. (2001) Solving a 300 kDa multimeric protein by low-resolution MAD phasing and averaging/phase extension. Acta Cryst. D 57:800.
• Yonath et al. (1998) Crystallographic studies on the ribosome, a large macromolecular assembly exhibiting severe nonisomorphism, extreme beam sensitivity and no internal symmetry. Acta Cryst. A 54:945.
• Knäblein et al. (1997) Ta6Br122+, a tool for phase determination of large biological assemblies by X-ray crystallography. J. Mol. Biol. 270:1.

• De et al. (2011) Crystal structure of the Vibrio cholerae cytolysin heptamer reveals common features among disparate pore-forming toxins. PNAS 108(18):7385.
• Spinelli et al. (2012) Crystal structure of Apis mellifera OBP14, a C-minus odorant-binding protein, and its complexes with odorant molecules. Insect Biochemistry and Molecular Biology 42(1):41.

Please contact xtals@jenabioscience.com with questions or inquiries.