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LEXSY Expression Kits - LEXSinduce2 Kit |
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The LEXSinduce2 Expression Kit yielded the highest expression levels of target proteins obtained so far. Protein expression in the engineered LEXSY host T7-TR is efficiently regulated by the well-known T7 RNA polymerase/TET repressor architecture. High-level transcription is induced by addition of tetracycline to the growth medium. The standard kit contains the vector pLEXSY_I-neo2 for selection of LEXSY T7-TR expression strains with the antibiotic LEXSY Neo.
Besides the neo marker, a vector with ble marker for selection with the antibiotic LEXSY Bleo is available. Expression vectors with both alternative markers can be introduced into the same cell for coexpression of two proteins.
Both vectors are also available separately. Links to their maps and sequences, are found in the
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 List of all LEXSY vectors: |
 View the Illustration of Inducible LEXSY here: |
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Downloads |
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LEXSinduce2 Expression Kit
contains vector pLEXSY_I-neo2
for inducible cytosolic or secretory expression |
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EGE-1400neo |
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1 Kit |
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2.250,00 |
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LEXSinduce2 Expression Kit
contains vector pLEXSY_I-ble2
for inducible cytosolic or secretory expression |
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EGE-1400ble |
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1 Kit |
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2.250,00 |
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Each kit contains:
- pLEXSY_I-2 vector of choice
- LEXSY host T7-TR as glycerol stocks
- all components for preparation of 1 L LEXSY BHI medium
- all selective antibiotics for 1 L medium
- primer sets for insert sequencing and diagnostic PCR
- an easy-to-follow manual, see PDF file in "Downloads"
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We also provide all the molecular tools required for the construction of expression plasmids, such as
Please contact expression@jenabioscience.com with questions or inquiries.
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