The LEXSinduce Expression Kits yielded the highest expression levels of target proteins obtained so far. Protein expression in the engineered LEXSY host T7-TR is efficiently regulated by the well-known T7 RNA polymerase/TET repressor architecture. High-level transcription is induced by addition of Tetracycline to the growth medium. Selection is performed with the antibiotic LEXSY Neo or LEXSY Bleo. Expression vectors with both alternative markers can be introduced into the same cell for coexpression of two proteins.

The 3rd generation of inducible LEXSY now allows convenient co-expression of the protein of interest with BleCherry conferring both, antibiotic resistance for selection with the antibiotic LEXSY Bleo as well as visible fluorescence under daylight as a direct read out for protein production. In addition, the new pLEXSY_I-3 vector generation has been reduced in size by about 1 kb compared to the 2nd generation vectors which allows easier cloning of large target genes (> 5 kbp) for expression.

All vectors are available separately. Links to their maps and sequences, are found in the

   List of all LEXSY vectors:

   View the General Illustration of Inducible LEXSY here:

   View the Illustration of 3rd Generation Inducible LEXSY with monitoring induction here:

Each kit contains:

• pLEXSY_I vector of choice
• LEXSY host T7-TR as glycerol stocks
• all components for preparation of 1 L LEXSY BHI medium
• all selective antibiotics for 1 L medium
• primer sets for insert sequencing and diagnostic PCR
• an easy-to-follow manual, see PDF file in "Downloads"

We also provide all the molecular tools required for the construction of expression plasmids, such as

• PCR tools, DNA isolation, purification and sequencing tools
• restriction enzymes, ligases

Please contact expression@jenabioscience.com with questions or inquiries.