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LEXSY Expression Kits - LEXSYcon-2 Kit |
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The LEXSYcon2 Expression Kit is the system of choice for most proteins. Dependent upon cloning strategy target proteins are either steadily expressed in the cytoplasm of the host or secreted into the growth medium. Cytosolically expressed proteins can be isolated from easily disrupted cells, whereas secreted proteins are efficiently isolated from the medium by one-step affinity chromatography. The standard kit contains the vector pLEXSY-sat2 for selection of LEXSY P10 expression strains with the antibiotic LEXSY NTC.
Besides the sat marker, vectors with three other selection markers (ble, hyg, neo) are available. Expression vectors with all four alternative markers can be introduced into the same cell for coexpression of up to four proteins. Please, indicate the marker of your choice in the comments field at the electronic order checkout, after the Cat.-No. EGE-1300... by Fax orders, or contact us by email: expression@jenabioscience.com
All vectors are also available separately. Links to their maps and sequences, are found in the
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 List of all LEXSY vectors |
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LEXSYcon2 Expression Kit
contains vector pLEXSY-sat2
for constitutive cytosolic or secretory expression |
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EGE-1300 |
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1 Kit |
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960,00 |
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Each kit contains:
- pLEXSY-2 vector of choice
- LEXSY host P10 as glycerol stocks
- all components for preparation of 1 L LEXSY BHI medium
- selective antibiotic for 1 L medium
- primer sets for insert sequencing and diagnostic PCR
- an easy-to-follow manual, see PDF file in "Downloads"
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We also provide all the molecular tools required for the construction of expression plasmids, such as
Please contact expression@jenabioscience.com with questions or inquiries.
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