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>
EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Sac I
5
'
-
G
A
G
C
T
|
C
-
3
'

3
'
-
C
|
T
C
G
A
G
-
5
'
AGCT-3'Sst I
Psp124B IEcl136 II, EcoICR I
Sac II
5
'
-
C
C
G
C
|
G
G
-
3
'

3
'
-
G
G
|
C
G
C
C
-
5
'
GC-3'SgrB I
Cfr42 I, Ksp I, Sfr303 I

Sal I
5
'
-
G
|
T
C
G
A
C
-
3
'

3
'
-
C
A
G
C
T
|
G
-
5
'
5'-TCGA



Sau3A I
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCMbo IDpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstENI I, BstMB I, Dpn II, Kzo9 I, Nde IIBstKT I
Sau96 I
5
'
-
G
|
G
N
C
C
-
3
'

3
'
-
C
C
N
G
|
G
-
5
'
5'-GNCPspP I
AspS9 I, Cfr13 I
Sca I
5
'
-
A
G
T
|
A
C
T
-
3
'

3
'
-
T
C
A
|
T
G
A
-
5
'
blunt

Acc113 I, Zrm I
Sfi I
5
'
-
G
G
C
C
N
N
N
N
|
N
G
G
C
C
-
3
'

3
'
-
C
C
G
G
N
|
N
N
N
N
C
C
G
G
-
5
'
NNN-3'



Sfo I
5
'
-
G
G
C
|
G
C
C
-
3
'

3
'
-
C
C
G
|
C
G
G
-
5
'
blunt
Nar I

5
'
-
G
G
|
C
G
C
C
-
3
'

3
'
-
C
C
G
C
|
G
G
-
5
'
Din I, Ege I, Ehe IBbe I, Kas I, Mly113 I
Sfr274 I
5
'
-
C
|
T
C
G
A
G
-
3
'

3
'
-
G
A
G
C
T
|
C
-
5
'
5'-TCGASla I
BssH I, PaeR7 I, Tli I, Xho I
Sfr303 I
5
'
-
C
C
G
C
|
G
G
-
3
'

3
'
-
G
G
|
C
G
C
C
-
5
'
GC-3'SgrB I
Cfr42 I, Ksp I, Sac II

Sfu I
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V
SgrB I
5
'
-
C
C
G
C
|
G
G
-
3
'

3
'
-
G
G
|
C
G
C
C
-
5
'
GC-3'

Cfr42 I, Ksp I, Sac II, Sfr303 I
Sin I
5
'
-
G
|
G
W
C
C
-
3
'

3
'
-
C
C
W
G
|
G
-
5
'
5'-GWCAva II
Afl I, Bme18 I, Eco47 I, VpaK11B I
Sla I
5
'
-
C
|
T
C
G
A
G
-
3
'

3
'
-
G
A
G
C
T
|
C
-
5
'
5'-TCGA

BssH I, PaeR7 I, Sfr274 I, Tli I, Xho I
Sma I
5
'
-
C
C
C
|
G
G
G
-
3
'

3
'
-
G
G
G
|
C
C
C
-
5
'
blunt


Cfr9 I, PspA I, Xma I, XmaC I
SnaB I
5
'
-
T
A
C
|
G
T
A
-
3
'

3
'
-
A
T
G
|
C
A
T
-
5
'
blunt

BstSN I, Eco105 I
SpaH I
5
'
-
G
C
A
T
G
|
C
-
3
'

3
'
-
C
|
G
T
A
C
G
-
5
'
CATG-3'Sph I
Bbu I, Pae I
Spe I
5
'
-
A
|
C
T
A
G
T
-
3
'

3
'
-
T
G
A
T
C
|
A
-
5
'
5'-CTAG

Ahl I, Bcu I
Sph I
5
'
-
G
C
A
T
G
|
C
-
3
'

3
'
-
C
|
G
T
A
C
G
-
5
'
CATG-3'

Bbu I, Pae I, SpaH I
SseB I
5
'
-
A
G
G
|
C
C
T
-
3
'

3
'
-
T
C
C
|
G
G
A
-
5
'
blunt

Aat I, Eco147 I, Pce I, Stu I
Ssp I
5
'
-
A
A
T
|
A
T
T
-
3
'

3
'
-
T
T
A
|
T
A
A
-
5
'
blunt



Sst I
5
'
-
G
A
G
C
T
|
C
-
3
'

3
'
-
C
|
T
C
G
A
G
-
5
'
AGCT-3'

Psp124B I, Sac IEcl136 II, EcoICR I
Stu I
5
'
-
A
G
G
|
C
C
T
-
3
'

3
'
-
T
C
C
|
G
G
A
-
5
'
bluntSseB I
Aat I, Eco147 I, Pce I
Sty I
5
'
-
C
|
C
W
W
G
G
-
3
'

3
'
-
G
G
W
W
C
|
C
-
5
'
5'-CWWG

BssT1 I, Eco130 I, EcoT14 I, Erh I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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