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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Pae I
5
'
-
G
 
C
A
T
G
|
C
-
3
'
 
3
'
-
C
|
G
T
A
C
 
G
-
5
'
CATG-3'Sph I
Bbu I, SpaH I
PaeR7 I
5
'
-
C
|
T
C
G
A
 
G
-
3
'
 
3
'
-
G
 
A
G
C
T
|
C
-
5
'
5'-TCGASla I
BssH I, Sfr274 I, Tli I, Xho I
Pal I
5
'
-
G
G
|
C
C
-
3
'
 
3
'
-
C
C
|
G
G
-
5
'
bluntBshF I
BspAN I, BsuR I, Hae III, Pho I
Pau I
5
'
-
G
|
C
G
C
G
 
C
-
3
'
 
3
'
-
C
 
G
C
G
C
|
G
-
5
'
5'-CGCGBssH II
BseP I
Pce I
5
'
-
A
G
G
|
C
C
T
-
3
'
 
3
'
-
T
C
C
|
G
G
A
-
5
'
bluntSseB I
Aat I, Eco147 I, Stu I
Pdi I
5
'
-
G
C
C
|
G
G
C
-
3
'
 
3
'
-
C
G
G
|
C
C
G
-
5
'
bluntNae I



MroN I, NgoM IV
PflF I
5
'
-
G
A
C
N
|
N
 
N
G
T
C
-
3
'
 
3
'
-
C
T
G
N
 
N
|
N
C
A
G
-
5
'
5'-NTth111 I
Asp I, Psy I, Tel I
Pho I
5
'
-
G
G
|
C
C
-
3
'
 
3
'
-
C
C
|
G
G
-
5
'
bluntBshF I
BspAN I, BsuR I, Hae III, Pal I
PinA I
5
'
-
A
|
C
C
G
G
 
T
-
3
'
 
3
'
-
T
 
G
G
C
C
|
A
-
5
'
5'-CCGGCspA I
Age I, AsiA I, BshT I
Ple19 I
5
'
-
C
G
 
A
T
|
C
G
-
3
'
 
3
'
-
G
C
|
T
A
 
G
C
-
5
'
AT-3'Pvu I
BpvU I, BspC I, Mvr I
Psp124B I
5
'
-
G
 
A
G
C
T
|
C
-
3
'
 
3
'
-
C
|
T
C
G
A
 
G
-
5
'
AGCT-3'Sst I
Sac IEcl136 II, EcoICR I
Psp6 I
5
'
-
|
C
C
W
G
G
 
-
3
'
 
3
'
-
 
G
G
W
C
C
|
-
5
'
5'-CCWGG
BseB I

5
'
-
C
C
|
W
 
G
G
-
3
'
 
3
'
-
G
G
 
W
|
C
C
-
5
'
Ajn I, EcoR II, PspG IBstN I, BstO I, Bst2U I, Mva I
PspA I
5
'
-
C
|
C
C
G
G
 
G
-
3
'
 
3
'
-
G
 
G
G
C
C
|
C
-
5
'
5'-CCGG
Sma I

5
'
-
C
C
C
|
G
G
G
-
3
'
 
3
'
-
G
G
G
|
C
C
C
-
5
'
Cfr9 I, Xma I, XmaC I
PspE I
5
'
-
G
|
G
T
N
A
C
 
C
-
3
'
 
3
'
-
C
 
C
A
N
T
G
|
G
-
5
'
5'-GTNACBstE II
BstP I, Eco91 I, EcoO65 I
PspG I
5
'
-
|
C
C
W
G
G
 
-
3
'
 
3
'
-
 
G
G
W
C
C
|
-
5
'
5'-CCWGG
BseB I

5
'
-
C
C
|
W
 
G
G
-
3
'
 
3
'
-
G
G
 
W
|
C
C
-
5
'
Ajn I, EcoR II, Psp6 IBstN I, BstO I, Bst2U I, Mva I
PspOM I
5
'
-
G
|
G
G
C
C
 
C
-
3
'
 
3
'
-
C
 
C
C
G
G
|
G
-
5
'
5'-GGCC
Apa I

5
'
-
G
 
G
G
C
C
|
C
-
3
'
 
3
'
-
C
|
C
C
G
G
 
G
-
5
'
Bsp120 I
PspP I
5
'
-
G
|
G
N
C
 
C
-
3
'
 
3
'
-
C
 
C
N
G
|
G
-
5
'
5'-GNC

AspS9 I, Cfr13 I, Sau96 I
Pst I
5
'
-
C
 
T
G
C
A
|
G
-
3
'
 
3
'
-
G
|
A
C
G
T
 
C
-
5
'
TGCA-3'

BstMA I
Psy I
5
'
-
G
A
C
N
|
N
 
N
G
T
C
-
3
'
 
3
'
-
C
T
G
N
 
N
|
N
C
A
G
-
5
'
5'-NTth111 I
Asp I, PflF I, Tel I
Pvu I
5
'
-
C
G
 
A
T
|
C
G
-
3
'
 
3
'
-
G
C
|
T
A
 
G
C
-
5
'
AT-3'

BpvU I, BspC I, Mvr I, Ple19 I,
Pvu II
5
'
-
C
A
G
|
C
T
G
-
3
'
 
3
'
-
G
T
C
|
G
A
C
-
5
'
blunt




 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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