A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Pae I	5 ' - G   C A T G | C - 3 '   3 ' - C | G T A C   G - 5 '	CATG-3'	Sph I		Bbu I, SpaH I
PaeR7 I	5 ' - C | T C G A   G - 3 '   3 ' - G   A G C T | C - 5 '	5'-TCGA	Sla I		BssH I, Sfr274 I, Tli I, Xho I
Pal I	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt	BshF I		BspAN I, BsuR I, Hae III, Pho I
Pau I	5 ' - G | C G C G   C - 3 '   3 ' - C   G C G C | G - 5 '	5'-CGCG	BssH II		BseP I
Pce I	5 ' - A G G | C C T - 3 '   3 ' - T C C | G G A - 5 '	blunt	SseB I		Aat I, Eco147 I, Stu I
Pdi I	5 ' - G C C | G G C - 3 '   3 ' - C G G | C C G - 5 '	blunt	Nae I			MroN I, NgoM IV
PflF I	5 ' - G A C N | N   N G T C - 3 '   3 ' - C T G N   N | N C A G - 5 '	5'-N	Tth111 I		Asp I, Psy I, Tel I
Pho I	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt	BshF I		BspAN I, BsuR I, Hae III, Pal I
PinA I	5 ' - A | C C G G   T - 3 '   3 ' - T   G G C C | A - 5 '	5'-CCGG	CspA I		Age I, AsiA I, BshT I
Ple19 I	5 ' - C G   A T | C G - 3 '   3 ' - G C | T A   G C - 5 '	AT-3'	Pvu I		BpvU I, BspC I, Mvr I
Psp124B I	5 ' - G   A G C T | C - 3 '   3 ' - C | T C G A   G - 5 '	AGCT-3'	Sst I		Sac I	Ecl136 II, EcoICR I
Psp6 I	5 ' - | C C W G G   - 3 '   3 ' -   G G W C C | - 5 '	5'-CCWGG		BseB I 5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	Ajn I, EcoR II, PspG I	BstN I, BstO I, Bst2U I, Mva I
PspA I	5 ' - C | C C G G   G - 3 '   3 ' - G   G G C C | C - 5 '	5'-CCGG		Sma I 5 ' - C C C | G G G - 3 '   3 ' - G G G | C C C - 5 '	Cfr9 I, Xma I, XmaC I
PspE I	5 ' - G | G T N A C   C - 3 '   3 ' - C   C A N T G | G - 5 '	5'-GTNAC	BstE II		BstP I, Eco91 I, EcoO65 I
PspG I	5 ' - | C C W G G   - 3 '   3 ' -   G G W C C | - 5 '	5'-CCWGG		BseB I 5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	Ajn I, EcoR II, Psp6 I	BstN I, BstO I, Bst2U I, Mva I
PspOM I	5 ' - G | G G C C   C - 3 '   3 ' - C   C C G G | G - 5 '	5'-GGCC		Apa I 5 ' - G   G G C C | C - 3 '   3 ' - C | C C G G   G - 5 '	Bsp120 I
PspP I	5 ' - G | G N C   C - 3 '   3 ' - C   C N G | G - 5 '	5'-GNC			AspS9 I, Cfr13 I, Sau96 I
Pst I	5 ' - C   T G C A | G - 3 '   3 ' - G | A C G T   C - 5 '	TGCA-3'			BstMA I
Psy I	5 ' - G A C N | N   N G T C - 3 '   3 ' - C T G N   N | N C A G - 5 '	5'-N	Tth111 I		Asp I, PflF I, Tel I
Pvu I	5 ' - C G   A T | C G - 3 '   3 ' - G C | T A   G C - 5 '	AT-3'			BpvU I, BspC I, Mvr I, Ple19 I,
Pvu II	5 ' - C A G | C T G - 3 '   3 ' - G T C | G A C - 5 '	blunt

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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