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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Pae I | 5 ' - G C A T G | C - 3 ' 3 ' - C | G T A C G - 5 ' | CATG-3' | Sph I |
| Bbu I, SpaH I |
| | PaeR7 I | 5 ' - C | T C G A G - 3 ' 3 ' - G A G C T | C - 5 ' | 5'-TCGA | Sla I |
| BssH I, Sfr274 I, Tli I, Xho I |
| | Pal I | | blunt | BshF I |
| BspAN I, BsuR I, Hae III, Pho I |
| | Pce I | 5 ' - A G G | C C T - 3 ' 3 ' - T C C | G G A - 5 ' | blunt | SseB I |
| Aat I, Eco147 I, Stu I |
| | Pci I | 5 ' - A | C A T G T - 3 ' 3 ' - T G T A C | A - 5 ' | 5'-CATG | BspLU11 I |
| Psc I |
| | Pdi I | 5 ' - G C C | G G C - 3 ' 3 ' - C G G | C C G - 5 ' | blunt | Nae I
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| MroN I, NgoM IV | | Pho I | | blunt | BshF I |
| BspAN I, BsuR I, Hae III, Pal I |
| | PinA I | 5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 ' | 5'-CCGG | CspA I |
| Age I, AsiA I, BshT I |
| | Psc I | 5 ' - A | C A T G T - 3 ' 3 ' - T G T A C | A - 5 ' | 5'-CATG | BspLU11 I |
| Pci I |
| | Psp124B I | 5 ' - G A G C T | C - 3 ' 3 ' - C | T C G A G - 5 ' | AGCT-3' | Sst I |
| Sac I | Ecl136 II, EcoICR I | | Psp6 I | 5 ' - | C C W G G - 3 ' 3 ' - G G W C C | - 5 ' | 5'-CCWGG |
| BseB I
5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 ' | Ajn I, EcoR II, PspG I | BstN I, BstO I, Bst2U I, Mva I | | PspA I | 5 ' - C | C C G G G - 3 ' 3 ' - G G G C C | C - 5 ' | 5'-CCGG |
| Sma I
5 ' - C C C | G G G - 3 ' 3 ' - G G G | C C C - 5 ' | Cfr9 I, Xma I, XmaC I |
| | PspE I | 5 ' - G | G T N A C C - 3 ' 3 ' - C C A N T G | G - 5 ' | 5'-GTNAC | BstE II |
| BstP I, Eco91 I, EcoO65 I |
| | PspG I | 5 ' - | C C W G G - 3 ' 3 ' - G G W C C | - 5 ' | 5'-CCWGG |
| BseB I
5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 ' | Ajn I, EcoR II, Psp6 I | BstN I, BstO I, Bst2U I, Mva I | | PspP I | 5 ' - G | G N C C - 3 ' 3 ' - C C N G | G - 5 ' | 5'-GNC |
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| AspS9 I, Cfr13 I, Sau96 I |
| | Pst I | 5 ' - C T G C A | G - 3 ' 3 ' - G | A C G T C - 5 ' | TGCA-3' |
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| BstMA I |
| | Pvu II | 5 ' - C A G | C T G - 3 ' 3 ' - G T C | G A C - 5 ' | blunt |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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