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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Kpn I | 5 ' - G G T A C | C - 3 ' 3 ' - C | C A T G G - 5 ' | GTAC-3' |
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| Acc65 I, Asp718 I | | Kpn2 I | 5 ' - T | C C G G A - 3 ' 3 ' - A G G C C | T - 5 ' | 5'-CCGG | BseA I |
| Acc III, Bsp13 I, BspE I, Mro I |
| | Ksp I | 5 ' - C C G C | G G - 3 ' 3 ' - G G | C G C C - 5 ' | GC-3' | SgrB I |
| Cfr42 I, Sac II, Sfr303 I |
| | Ksp22 I | 5 ' - T | G A T C A - 3 ' 3 ' - A C T A G | T - 5 ' | 5'-GATC | Bcl I |
| Fba I |
| | KspA I | 5 ' - G T T | A A C - 3 ' 3 ' - C A A | T T G - 5 ' | blunt | Hpa I |
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| | Kzo9 I | 5 ' - | G A T C - 3 ' 3 ' - C T A G | - 5 ' | 5'-GATC | Mbo I
Sau3A I | Dpn I
| BfuCI, Bsp143 I, BstEN II, BstMB I, Dpn II, Nde II
| BstKT I |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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