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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Kas I
5
'
-
G
|
G
C
G
C
 
C
-
3
'
 
3
'
-
C
 
C
G
C
G
|
G
-
5
'
5'GCGC
Nar I

5
'
-
G
G
|
C
G
 
C
C
-
3
'
 
3
'
-
C
C
 
G
C
|
G
G
-
5
'

Bbe I, Din I, Ege I, Ehe I, Mly113 I, Sfo I
Kpn I
5
'
-
G
 
G
T
A
C
|
C
-
3
'
 
3
'
-
C
|
C
A
T
G
 
G
-
5
'
GTAC-3'


Acc65 I, Asp718 I
Kpn2 I
5
'
-
T
|
C
C
G
G
 
A
-
3
'
 
3
'
-
A
 
G
G
C
C
|
T
-
5
'
5'-CCGGBseA I
Acc III, Bsp13 I, BspE I, Mro I
Ksp I
5
'
-
C
C
 
G
C
|
G
G
-
3
'
 
3
'
-
G
G
|
C
G
 
C
C
-
5
'
GC-3'SgrB I
Cfr42 I, Sac II, Sfr303 I
Ksp22 I
5
'
-
T
|
G
A
T
C
 
A
-
3
'
 
3
'
-
A
 
C
T
A
G
|
T
-
5
'
5'-GATCBcl I
Fba I
KspA I
5
'
-
G
T
T
|
A
A
C
-
3
'
 
3
'
-
C
A
A
|
T
T
G
-
5
'
bluntHpa I


Kzo9 I
5
'
-
|
G
A
T
C
 
-
3
'
 
3
'
-
 
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'
 
3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Nde II
BstKT I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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