A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Kas I	5 ' - G | G C G C   C - 3 '   3 ' - C   C G C G | G - 5 '	5'GCGC		Nar I 5 ' - G G | C G   C C - 3 '   3 ' - C C   G C | G G - 5 '		Bbe I, Din I, Ege I, Ehe I, Mly113 I, Sfo I
Kpn I	5 ' - G   G T A C | C - 3 '   3 ' - C | C A T G   G - 5 '	GTAC-3'				Acc65 I, Asp718 I
Kpn2 I	5 ' - T | C C G G   A - 3 '   3 ' - A   G G C C | T - 5 '	5'-CCGG	BseA I		Acc III, Bsp13 I, BspE I, Mro I
Ksp I	5 ' - C C   G C | G G - 3 '   3 ' - G G | C G   C C - 5 '	GC-3'	SgrB I		Cfr42 I, Sac II, Sfr303 I
Ksp22 I	5 ' - T | G A T C   A - 3 '   3 ' - A   C T A G | T - 5 '	5'-GATC	Bcl I		Fba I
KspA I	5 ' - G T T | A A C - 3 '   3 ' - C A A | T T G - 5 '	blunt	Hpa I
Kzo9 I	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Nde II	BstKT I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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