A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Hae III	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt	BshF I		BspAN I, BsuR I, Pal I, Pho I
Hap II	5 ' - C | C G   G - 3 '   3 ' - G   G C | C - 5 '	5'-CG	BsiS I		Hpa II, Msp I
Hinc II	5 ' - G T Y | R A C - 3 '   3 ' - C A R | Y T G - 5 '	blunt			Hind II
Hind II	5 ' - G T Y | R A C - 3 '   3 ' - C A R | Y T G - 5 '	blunt	Hinc II
Hind III	5 ' - A | A G C T   T - 3 '   3 ' - T   T C G A | A - 5 '	5'-AGCT
Hinf I	5 ' - G | A N T   C - 3 '   3 ' - C   T N A | G - 5 '	5'-ANT
Hpa I	5 ' - G T T | A A C - 3 '   3 ' - C A A | T T G - 5 '	blunt			KspA I
Hpa II	5 ' - C | C G   G - 3 '   3 ' - G   G C | C - 5 '	5'-CG	BsiS I		Hap II, Msp I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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