A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z


 
EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Hae III
5
'
-
G
G
|
C
C
-
3
'

3
'
-
C
C
|
G
G
-
5
'
bluntBshF I
BspAN I, BsuR I, Pal I, Pho I
Hap II
5
'
-
C
|
C
G
G
-
3
'

3
'
-
G
G
C
|
C
-
5
'
5'-CGHpa II
BsiS I, Msp I
Hinc II
5
'
-
G
T
Y
|
R
A
C
-
3
'

3
'
-
C
A
R
|
Y
T
G
-
5
'
blunt

Hind II
Hind II
5
'
-
G
T
Y
|
R
A
C
-
3
'

3
'
-
C
A
R
|
Y
T
G
-
5
'
bluntHinc II


Hind III
5
'
-
A
|
A
G
C
T
T
-
3
'

3
'
-
T
T
C
G
A
|
A
-
5
'
5'-AGCT



Hinf I
5
'
-
G
|
A
N
T
C
-
3
'

3
'
-
C
T
N
A
|
G
-
5
'
5'-ANT



Hpa I
5
'
-
G
T
T
|
A
A
C
-
3
'

3
'
-
C
A
A
|
T
T
G
-
5
'
blunt

KspA I
Hpa II
5
'
-
C
|
C
G
G
-
3
'

3
'
-
G
G
C
|
C
-
5
'
5'-CGBsiS I
Hap II, Msp I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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