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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Ecl136 II | 5 ' - G A G | C T C - 3 ' 3 ' - C T C | G A G - 5 ' | blunt |
| Sst I
5 ' - G A G C T | C - 3 ' 3 ' - C | T C G A G - 5 ' | EcoICR I | Psp124B I, Sac I | | Eco105 I | 5 ' - T A C | G T A - 3 ' 3 ' - A T G | C A T - 5 ' | blunt | SnaB I |
| BstSN I |
| | Eco130 I | 5 ' - C | C W W G G - 3 ' 3 ' - G G W W C | C - 5 ' | 5'-CWWG | Sty I |
| BssT1 I, EcoT14 I, Erh I |
| | Eco147 I | 5 ' - A G G | C C T - 3 ' 3 ' - T C C | G G A - 5 ' | blunt | SseB I |
| Aat I, Pce I, Stu I |
| | Eco32 I | 5 ' - G A T | A T C - 3 ' 3 ' - C T A | T A G - 5 ' | blunt | EcoR V |
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| | Eco91 I | 5 ' - G | G T N A C C - 3 ' 3 ' - C C A N T G | G - 5 ' | 5'-GTNAC | BstE II |
| BstP I, EcoO65 I, PspE I |
| | EcoICR I | 5 ' - G A G | C T C - 3 ' 3 ' - C T C | G A G - 5 ' | blunt |
| Sst I
5 ' - G A G C T | C - 3 ' 3 ' - C | T C G A G - 5 ' | Ecl136I I | Psp124B I, Sac I | | EcoO65 I | 5 ' - G | G T N A C C - 3 ' 3 ' - C C A N T G | G - 5 ' | 5'-GTNAC | BstE II |
| BstP I, Eco91 I, PspE I |
| | EcoR I | 5 ' - G | A A T T C - 3 ' 3 ' - C T T A A | G - 5 ' | 5'-AATT |
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| FunII |
| | EcoR II | 5 ' - | C C W G G - 3 ' 3 ' - G G W C C | - 5 ' | 5'-CCWGG |
| BseB I
5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 ' | Ajn I, Psp6 I, PspG I | BstN I, BstO I, Bst2U I, Mva I | | EcoR V | 5 ' - G A T | A T C - 3 ' 3 ' - C T A | T A G - 5 ' | blunt |
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| Eco32 I |
| | EcoT14 I | 5 ' - C | C W W G G - 3 ' 3 ' - G G W W C | C - 5 ' | 5'-CWWG | Sty I |
| BssT1 I, Eco130 I, Erh I |
| | Erh I | 5 ' - C | C W W G G - 3 ' 3 ' - G G W W C | C - 5 ' | 5'-CWWG | Sty I |
| BssT1 I, Eco130 I, EcoT14 I |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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