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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)		IsoNeo
Din I
5
'
-
G
G
C
|
G
C
C
-
3
'
 
3
'
-
C
C
G
|
C
G
G
-
5
'
blunt
Nar I

5
'
-
G
G
|
C
G
 
C
C
-
3
'
 
3
'
-
C
C
 
G
C
|
G
G
-
5
'
Ege I, Ehe I, Sfo I Bbe I, Kas I, Mly113 I
Dpn I
5
'
-
G
A
|
T
C
-
3
'
 
3
'
-
C
T
|
A
G
-
5
'
blunt
Mbo I Sau3A I

5
'
-
|
G
A
T
C
 
-
3
'
 
3
'
-
 
C
T
A
G
|
-
5
'

BfuC I, Bsp143 I, BstEN II, BstMB I, BstKT I, Dpn II, Kzo9 I, Nde II
Dpn II
5
'
-
|
G
A
T
C
 
-
3
'
 
3
'
-
 
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I		Dpn I

5
'
-
G
A
|
T
C
-
3
'
 
3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstEN II, BstMB I, Kzo9 I, Nde IIBstKT I
Dra I
5
'
-
T
T
T
|
A
A
A
-
3
'
 
3
'
-
A
A
A
|
T
T
T
-
5
'
blunt

Aha III

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

Please contact enzymes@jenabioscience.com for more information.