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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | CciN I | 5 ' - G C | G G C C G C - 3 ' 3 ' - C G C C G G | C G - 5 ' | 5'-GGCC | Not I |
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| | Cfr10 I | 5 ' - R C | C G G Y - 3 ' 3 ' - Y G G C | C R - 5 ' | 5'-CG | BssA I |
| Bse118 I, BsrF I |
| | Cfr13 I | 5 ' - G | G N C C - 3 ' 3 ' - C C N G | G - 5 ' | 5'-GNC | PspP I |
| AspS9 I, Sau96 I |
| | Cfr42 I | 5 ' - C C G C | G G - 3 ' 3 ' - G G | C G C C - 5 ' | GC-3' | SgrB I |
| Ksp I, Sac II, Sfr303 I |
| | Cfr9 I | 5 ' - C | C C G G G - 3 ' 3 ' - G G G C C | C - 5 ' | 5'-CCGG |
| Sma I
5 ' - C C C | G G G - 3 ' 3 ' - G G G | C C C - 5 ' | PspAI, Xma I, XmaC I |
| | Cla I | 5 ' - A T | C G A T - 3 ' 3 ' - T A G C | T A - 5 ' | 5'-CG | BseC I |
| Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Zho I |
| | Csp45 I | 5 ' - T T | C G A A - 3 ' 3 ' - A A G C | T T - 5 ' | 5'-CG | Asu II |
| Bpu14 I, Bsp119 I, BspT104 I, BstB I, Nsp V, Sfu I |
| | Csp6 I | 5 ' - G | T A C - 3 ' 3 ' - C A T | G - 5 ' | 5'-TA |
| Rsa I
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| Afa I | | CspA I | 5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 ' | 5'-CCGG |
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| Age I, AsiA I, BshT I, PinA I |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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