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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
CciN I
5
'
-
G
C
|
G
G
C
C
 
G
C
-
3
'
 
3
'
-
C
G
C
C
 
G
G
|
C
G
-
5
'
5'-GGCCNot I


Cfr10 I
5
'
-
R
C
|
C
G
 
G
Y
-
3
'
 
3
'
-
Y
G
 
G
C
|
C
R
-
5
'
5'-CGBssA I
Bse118 I, BsrF I
Cfr13 I
5
'
-
G
|
G
N
C
 
C
-
3
'
 
3
'
-
C
 
C
N
G
|
G
-
5
'
5'-GNCPspP I
AspS9 I, Sau96 I
Cfr42 I
5
'
-
C
C
 
G
C
|
G
G
-
3
'
 
3
'
-
G
G
|
C
G
 
C
C
-
5
'
GC-3'SgrB I
Ksp I, Sac II, Sfr303 I
Cfr9 I
5
'
-
C
|
C
C
G
G
 
G
-
3
'
 
3
'
-
G
 
G
G
C
C
|
C
-
5
'
5'-CCGG
Sma I

5
'
-
C
C
C
|
G
G
G
-
3
'
 
3
'
-
G
G
G
|
C
C
C
-
5
'
PspA I, Xma I, XmaC I
Cla I
5
'
-
A
T
|
C
G
 
A
T
-
3
'
 
3
'
-
T
A
 
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Zho I
Cpo I
5
'
-
C
G
|
G
W
C
 
C
G
-
3
'
 
3
'
-
G
C
 
C
W
G
|
G
C
-
5
'
5'-GWCRsr II
Csp I, Rsr2 I
Csp I
5
'
-
C
G
|
G
W
C
 
C
G
-
3
'
 
3
'
-
G
C
 
C
W
G
|
G
C
-
5
'
5'-GWCRsr II
Cpo I, Rsr2 I
Csp45 I
5
'
-
T
T
|
C
G
 
A
A
-
3
'
 
3
'
-
A
A
 
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, Bsp119 I, BspT104 I, BstB I, Nsp V, Sfu I
Csp6 I
5
'
-
G
|
T
A
 
C
-
3
'
 
3
'
-
C
 
A
T
|
G
-
5
'
5'-TA
Rsa I

5
'
-
G
T
|
A
C
-
3
'
 
3
'
-
C
A
|
T
G
-
5
'

Afa I
CspA I
5
'
-
A
|
C
C
G
G
 
T
-
3
'
 
3
'
-
T
 
G
G
C
C
|
A
-
5
'
5'-CCGG

Age I, AsiA I, BshT I, PinA I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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