A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
CciN I	5 ' - G C | G G C C   G C - 3 '   3 ' - C G C C   G G | C G - 5 '	5'-GGCC	Not I
Cfr10 I	5 ' - R C | C G   G Y - 3 '   3 ' - Y G   G C | C R - 5 '	5'-CG	BssA I		Bse118 I, BsrF I
Cfr13 I	5 ' - G | G N C   C - 3 '   3 ' - C   C N G | G - 5 '	5'-GNC	PspP I		AspS9 I, Sau96 I
Cfr42 I	5 ' - C C   G C | G G - 3 '   3 ' - G G | C G   C C - 5 '	GC-3'	SgrB I		Ksp I, Sac II, Sfr303 I
Cfr9 I	5 ' - C | C C G G   G - 3 '   3 ' - G   G G C C | C - 5 '	5'-CCGG		Sma I 5 ' - C C C | G G G - 3 '   3 ' - G G G | C C C - 5 '	PspA I, Xma I, XmaC I
Cla I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Zho I
Cpo I	5 ' - C G | G W C   C G - 3 '   3 ' - G C   C W G | G C - 5 '	5'-GWC	Rsr II		Csp I, Rsr2 I
Csp I	5 ' - C G | G W C   C G - 3 '   3 ' - G C   C W G | G C - 5 '	5'-GWC	Rsr II		Cpo I, Rsr2 I
Csp45 I	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bpu14 I, Bsp119 I, BspT104 I, BstB I, Nsp V, Sfu I
Csp6 I	5 ' - G | T A   C - 3 '   3 ' - C   A T | G - 5 '	5'-TA		Rsa I 5 ' - G T | A C - 3 '   3 ' - C A | T G - 5 '		Afa I
CspA I	5 ' - A | C C G G   T - 3 '   3 ' - T   G G C C | A - 5 '	5'-CCGG			Age I, AsiA I, BshT I, PinA I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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