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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Nae I
5
'
-
G
C
C
|
G
G
C
-
3
'
 
3
'
-
C
G
G
|
C
C
G
-
5
'
blunt

Pdi IMroN I, NgoM IV
Nar I
5
'
-
G
G
|
C
G
 
C
C
-
3
'
 
3
'
-
C
C
 
G
C
|
G
G
-
5
'
5'-CG

Mly113 IBbe I, Din I, Ege I, Ehe I, Sfo I, Kas I
Nco I
5
'
-
C
|
C
A
T
G
 
G
-
3
'
 
3
'
-
G
 
G
T
A
C
|
C
-
5
'
5'-CATG

Bsp19 I
Nde I
5
'
-
C
A
|
T
A
 
T
G
-
3
'
 
3
'
-
G
T
 
A
T
|
A
C
-
5
'
5'-TA

FauND I
Nde II
5
'
-
|
G
A
T
C
 
-
3
'
 
3
'
-
 
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'
 
3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 IBstKT I
NgoM IV
5
'
-
G
|
C
C
G
G
 
C
-
3
'
 
3
'
-
C
 
G
G
C
C
|
G
-
5
'
5'-CCGG
Nae I

5
'
-
G
C
C
|
G
G
C
-
3
'
 
3
'
-
C
G
G
|
C
C
G
-
5
'
MroN IPdi I
Nhe I
5
'
-
G
|
C
T
A
G
 
C
-
3
'
 
3
'
-
C
 
G
A
T
C
|
G
-
5
'
5'-CTAG

AsuNH IBmt I
Not I
5
'
-
G
C
|
G
G
C
C
 
G
C
-
3
'
 
3
'
-
C
G
C
C
 
G
G
|
C
G
-
5
'
5'-GGCC

CciN I
Nru I
5
'
-
T
C
G
|
C
G
A
-
3
'
 
3
'
-
A
G
C
|
G
C
T
-
5
'
blunt

Bsp68 I
Nsp III
5
'
-
C
|
Y
C
G
R
 
G
-
3
'
 
3
'
-
G
 
R
G
C
Y
|
C
-
5
'
5'-YCGRAva I
Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 IBmeT110 I
Nsp V
5
'
-
T
T
|
C
G
 
A
A
-
3
'
 
3
'
-
A
A
 
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Sfu I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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