A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Nae I	5 ' - G C C | G G C - 3 '   3 ' - C G G | C C G - 5 '	blunt			Pdi I	MroN I, NgoM IV
Nar I	5 ' - G G | C G   C C - 3 '   3 ' - C C   G C | G G - 5 '	5'-CG			Mly113 I	Bbe I, Din I, Ege I, Ehe I, Sfo I, Kas I
Nco I	5 ' - C | C A T G   G - 3 '   3 ' - G   G T A C | C - 5 '	5'-CATG			Bsp19 I
Nde I	5 ' - C A | T A   T G - 3 '   3 ' - G T   A T | A C - 5 '	5'-TA			FauND I
Nde II	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I	BstKT I
NgoM IV	5 ' - G | C C G G   C - 3 '   3 ' - C   G G C C | G - 5 '	5'-CCGG		Nae I 5 ' - G C C | G G C - 3 '   3 ' - C G G | C C G - 5 '	MroN I	Pdi I
Nhe I	5 ' - G | C T A G   C - 3 '   3 ' - C   G A T C | G - 5 '	5'-CTAG			AsuNH I	Bmt I
Not I	5 ' - G C | G G C C   G C - 3 '   3 ' - C G C C   G G | C G - 5 '	5'-GGCC			CciN I
Nru I	5 ' - T C G | C G A - 3 '   3 ' - A G C | G C T - 5 '	blunt			Bsp68 I
Nsp III	5 ' - C | Y C G R   G - 3 '   3 ' - G   R G C Y | C - 5 '	5'-YCGR	Ava I		Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 I	BmeT110 I
Nsp V	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Sfu I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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