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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Nae I | 5 ' - G C C | G G C - 3 ' 3 ' - C G G | C C G - 5 ' | blunt |
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| Pdi I | MroN I, NgoM IV | | Nco I | 5 ' - C | C A T G G - 3 ' 3 ' - G G T A C | C - 5 ' | 5'-CATG |
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| Bsp19 I |
| | Nde II | 5 ' - | G A T C - 3 ' 3 ' - C T A G | - 5 ' | 5'-GATC | Mbo I
Sau3A I | Dpn I
| BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I | BstKT I | | NgoM IV | 5 ' - G | C C G G C - 3 ' 3 ' - C G G C C | G - 5 ' | 5'-CCGG |
| Nae I
5 ' - G C C | G G C - 3 ' 3 ' - C G G | C C G - 5 ' | MroN I | Pdi I | | Nhe I | 5 ' - G | C T A G C - 3 ' 3 ' - C G A T C | G - 5 ' | 5'-CTAG |
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| AsuNH I | Bmt I | | Not I | 5 ' - G C | G G C C G C - 3 ' 3 ' - C G C C G G | C G - 5 ' | 5'-GGCC |
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| CciN I |
| | Nru I | 5 ' - T C G | C G A - 3 ' 3 ' - A G C | G C T - 5 ' | blunt |
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| Bsp68 I |
| | Nsp V | 5 ' - T T | C G A A - 3 ' 3 ' - A A G C | T T - 5 ' | 5'-CG | Asu II |
| Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Sfu I |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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