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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Aat I | 5 ' - A G G | C C T - 3 ' 3 ' - T C C | G G A - 5 ' | blunt | SseB I |
| Eco147 I, Pce I, Stu I |
| | Acc III | 5 ' - T | C C G G A - 3 ' 3 ' - A G G C C | T - 5 ' | 5'-CCGG | BseA I |
| Bsp13 I, BspE I, Kpn2 I, Mro I |
| | Acc113 I | 5 ' - A G T | A C T - 3 ' 3 ' - T C A | T G A - 5 ' | blunt | Sca I |
| Zrm I |
| | Acc65 I | 5 ' - G | G T A C C - 3 ' 3 ' - C C A T G | G - 5 ' | 5'-GTAC |
| Kpn I
5 ' - G G T A C | C - 3 ' 3 ' - C | C A T G G - 5 ' | Asp718 I |
| | Afa I | | blunt | Rsa I |
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| Csp6 I | | Afl II | 5 ' - C | T T A A G - 3 ' 3 ' - G A A T T | C - 5 ' | 5'-TTAA | MspC I |
| Bfr I, BspT I, Bst98 I, Vha464 I |
| Age I
| 5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 ' | 5'-CCGG | CspA I |
| AsiAI, BshT I, PinA I |
| | Ajn I | 5 ' - | C C W G G - 3 ' 3 ' - G G W C C | - 5 ' | 5'-CCWGG |
| BseB I
5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 ' | EcoRII, Psp6 I, PspG I | BstNI, BstO I, Bst2U I, Mva I | Alu I
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| | Ama87 I | 5 ' - C | Y C G R G - 3 ' 3 ' - G R G C Y | C - 5 ' | 5'-YCGR | BspLU4 I |
| Ava I, BsiHKC I, BsoB I, Eco88 I, Nsp III |
| | Alw44 I | 5 ' - G | T G C A C - 3 ' 3 ' - C T C G T | G - 5 ' | 5'-TGCA | ApaL I |
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| | ApaL I | 5 ' - G | T G C A C - 3 ' 3 ' - C T C G T | G - 5 ' | 5'-TGCA |
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| Alw44 I |
| | AsiA I | 5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 ' | 5'-CCGG | CspA I |
| Age I, BshT I, PinA I |
| | Asp718 I | 5 ' - G | G T A C C - 3 ' 3 ' - C C A T G | G - 5 ' | 5'-GTAC |
| Kpn I
5 ' - G G T A C | C - 3 ' 3 ' - C | C A T G G - 5 ' | Acc65 I |
| AspS9 I
| 5 ' - G | G N C C - 3 ' 3 ' - C C N G | G - 5 ' | 5'-GNC | PspP I |
| Cfr13I, Sau96 I |
| | Asu II | 5 ' - T T | C G A A - 3 ' 3 ' - A A G C | T T - 5 ' | 5'-CG |
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| Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V,Sfu I |
| | AsuNH I | 5 ' - G | C T A G C - 3 ' 3 ' - C G A T C | G - 5 ' | 5'-CTAG | Nhe I |
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| Bmt I | | Ava I | 5 ' - C | Y C G R G - 3 ' 3 ' - G R G C Y | C - 5 ' | 5'-YCGR | BspLU4 I |
| Ama87 I, BsiHKC I, BsoB I, Eco88 I, Nsp III |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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