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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Aat I
5
'
-
A
G
G
|
C
C
T
-
3
'

3
'
-
T
C
C
|
G
G
A
-
5
'
bluntSseB I
Eco147 I, Pce I, Stu I
Acc I
5
'
-
G
T
|
M
K
A
C
-
3
'

3
'
-
C
A
K
M
|
T
G
-
5
'
5'-MK

Fbl I, Xmi I
Acc III
5
'
-
T
|
C
C
G
G
A
-
3
'

3
'
-
A
G
G
C
C
|
T
-
5
'
5'-CCGGBseA I
Bsp13 I, BspE I, Kpn2 I, Mro I
Acc113 I
5
'
-
A
G
T
|
A
C
T
-
3
'

3
'
-
T
C
A
|
T
G
A
-
5
'
bluntSca I
Zrm I
Acc65 I
5
'
-
G
|
G
T
A
C
C
-
3
'

3
'
-
C
C
A
T
G
|
G
-
5
'
5'-GTAC
Kpn I

5
'
-
G
G
T
A
C
|
C
-
3
'

3
'
-
C
|
C
A
T
G
G
-
5
'
Asp718 I
Afa I
5
'
-
G
T
|
A
C
-
3
'

3
'
-
C
A
|
T
G
-
5
'
bluntRsa I

Csp6 I
Afl I
5
'
-
G
|
G
W
C
C
-
3
'

3
'
-
C
C
W
G
|
G
-
5
'
5'-GWCAva II
Bme18 I, Eco47 I, Sin I, VpaK11B I
Afl II
5
'
-
C
|
T
T
A
A
G
-
3
'

3
'
-
G
A
A
T
T
|
C
-
5
'
5'-TTAAMspC I
Bfr I, BspT I, Bst98 I, Vha464 I
Age I
5
'
-
A
|
C
C
G
G
T
-
3
'

3
'
-
T
G
G
C
C
|
A
-
5
'
5'-CCGGCspA I
AsiA I, BshT I, PinA I
Aha III
5
'
-
T
T
T
|
A
A
A
-
3
'

3
'
-
A
A
A
|
T
T
T
-
5
'
bluntDra I


Ahl I
5
'
-
A
|
C
T
A
G
T
-
3
'

3
'
-
T
G
A
T
C
|
A
-
5
'
5'-CTAGSpe I
Bcu I
Ajn I
5
'
-
|
C
C
W
G
G
-
3
'

3
'
-
G
G
W
C
C
|
-
5
'
5'-CCWGG
BseB I

5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
EcoR II, Psp6 I, PspG IBstN I, BstO I, Bst2U I, Mva I
Alu I
5
'
-
A
G
|
C
T
-
3
'

3
'
-
T
C
|
G
A
-
5
'
blunt


Alw44 I
5
'
-
G
|
T
G
C
A
C
-
3
'

3
'
-
C
T
C
G
T
|
G
-
5
'
5'-TGCAApaL I


Ama87 I
5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'
5'-YCGRAva I
Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp IIIBmeT110 I
Apa I
5
'
-
G
G
G
C
C
|
C
-
3
'

3
'
-
C
|
C
C
G
G
G
-
5
'
GGGCC-3'


Bsp120 I, PspOM I
ApaL I
5
'
-
G
|
T
G
C
A
C
-
3
'

3
'
-
C
T
C
G
T
|
G
-
5
'
5'-TGCA

Alw44 I
AsiA I
5
'
-
A
|
C
C
G
G
T
-
3
'

3
'
-
T
G
G
C
C
|
A
-
5
'
5'-CCGGCspA I
Age I, BshT I, PinA I
Asp I
5
'
-
G
A
C
N
|
N
N
G
T
C
-
3
'

3
'
-
C
T
G
N
N
|
N
C
A
G
-
5
'
5'-NTth111 I
PflF I, Psy I, Tel I
Asp718 I
5
'
-
G
|
G
T
A
C
C
-
3
'

3
'
-
C
C
A
T
G
|
G
-
5
'
5'-GTAC
Kpn I

5
'
-
G
G
T
A
C
|
C
-
3
'

3
'
-
C
|
C
A
T
G
G
-
5
'
Acc65 I
AspS9 I
5
'
-
G
|
G
N
C
C
-
3
'

3
'
-
C
C
N
G
|
G
-
5
'
5'-GNCPspP I
Cfr13 I, Sau96 I
Asu II
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CG

Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
AsuNH I
5
'
-
G
|
C
T
A
G
C
-
3
'

3
'
-
C
G
A
T
C
|
G
-
5
'
5'-CTAGNhe I

Bmt I
Ava I
5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'
5'-YCGR

Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp IIIBmeT110 I
Ava II
5
'
-
G
|
G
W
C
C
-
3
'

3
'
-
C
C
W
G
|
G
-
5
'
5'-GWC

Afl I, Bme18 I, Eco47 I, Sin I, VpaK11B I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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