A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Aat I	5 ' - A G G | C C T - 3 ' 3 ' - T C C | G G A - 5 '	blunt	SseB I		Eco147 I, Pce I, Stu I
Acc I	5 ' - G T | M K A C - 3 ' 3 ' - C A K M | T G - 5 '	5'-MK			Fbl I, Xmi I
Acc III	5 ' - T | C C G G A - 3 ' 3 ' - A G G C C | T - 5 '	5'-CCGG	BseA I		Bsp13 I, BspE I, Kpn2 I, Mro I
Acc113 I	5 ' - A G T | A C T - 3 ' 3 ' - T C A | T G A - 5 '	blunt	Sca I		Zrm I
Acc65 I	5 ' - G | G T A C C - 3 ' 3 ' - C C A T G | G - 5 '	5'-GTAC		Kpn I 5 ' - G G T A C | C - 3 ' 3 ' - C | C A T G G - 5 '	Asp718 I
Afa I	5 ' - G T | A C - 3 ' 3 ' - C A | T G - 5 '	blunt	Rsa I			Csp6 I
Afl I	5 ' - G | G W C C - 3 ' 3 ' - C C W G | G - 5 '	5'-GWC	Ava II		Bme18 I, Eco47 I, Sin I, VpaK11B I
Afl II	5 ' - C | T T A A G - 3 ' 3 ' - G A A T T | C - 5 '	5'-TTAA	MspC I		Bfr I, BspT I, Bst98 I, Vha464 I
Age I	5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 '	5'-CCGG	CspA I		AsiA I, BshT I, PinA I
Aha III	5 ' - T T T | A A A - 3 ' 3 ' - A A A | T T T - 5 '	blunt	Dra I
Ahl I	5 ' - A | C T A G T - 3 ' 3 ' - T G A T C | A - 5 '	5'-CTAG	Spe I		Bcu I
Ajn I	5 ' - | C C W G G - 3 ' 3 ' - G G W C C | - 5 '	5'-CCWGG		BseB I 5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 '	EcoR II, Psp6 I, PspG I	BstN I, BstO I, Bst2U I, Mva I
Alu I	5 ' - A G | C T - 3 ' 3 ' - T C | G A - 5 '	blunt
Alw44 I	5 ' - G | T G C A C - 3 ' 3 ' - C T C G T | G - 5 '	5'-TGCA	ApaL I
Ama87 I	5 ' - C | Y C G R G - 3 ' 3 ' - G R G C Y | C - 5 '	5'-YCGR	Ava I		Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp III	BmeT110 I
Apa I	5 ' - G G G C C | C - 3 ' 3 ' - C | C C G G G - 5 '	GGGCC-3'				Bsp120 I, PspOM I
ApaL I	5 ' - G | T G C A C - 3 ' 3 ' - C T C G T | G - 5 '	5'-TGCA			Alw44 I
AsiA I	5 ' - A | C C G G T - 3 ' 3 ' - T G G C C | A - 5 '	5'-CCGG	CspA I		Age I, BshT I, PinA I
Asp I	5 ' - G A C N | N N G T C - 3 ' 3 ' - C T G N N | N C A G - 5 '	5'-N	Tth111 I		PflF I, Psy I, Tel I
Asp718 I	5 ' - G | G T A C C - 3 ' 3 ' - C C A T G | G - 5 '	5'-GTAC		Kpn I 5 ' - G G T A C | C - 3 ' 3 ' - C | C A T G G - 5 '	Acc65 I
AspS9 I	5 ' - G | G N C C - 3 ' 3 ' - C C N G | G - 5 '	5'-GNC	PspP I		Cfr13 I, Sau96 I
Asu II	5 ' - T T | C G A A - 3 ' 3 ' - A A G C | T T - 5 '	5'-CG			Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
AsuNH I	5 ' - G | C T A G C - 3 ' 3 ' - C G A T C | G - 5 '	5'-CTAG	Nhe I			Bmt I
Ava I	5 ' - C | Y C G R G - 3 ' 3 ' - G R G C Y | C - 5 '	5'-YCGR			Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp III	BmeT110 I
Ava II	5 ' - G | G W C C - 3 ' 3 ' - C C W G | G - 5 '	5'-GWC			Afl I, Bme18 I, Eco47 I, Sin I, VpaK11B I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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