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Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
BamH I	5 ' - G | G A T C   C - 3 '   3 ' - C   C T A G | G - 5 '	5'-GATC
Ban II	5 ' - G   R G C Y | C - 3 '   3 ' - C | Y C G R   G - 5 '	RGCY-3'			Eco24 I, EcoT38 I, FriO I
Ban III	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bbe I	5 ' - G   G C G C | C - 3 '   3 ' - C | C G C G   G - 5 '	GCGC-3'		Nar I 5 ' - G G | C G   C C - 3 '   3 ' - C C   G C | G G - 5 '		Din I, Ege I, Ehe I, Kas I, Mly113 I, Sfo I
Bbu I	5 ' - G   C A T G | C - 3 '   3 ' - C | G T A C   G - 5 '	CATG-3'	Sph I		Pae I, SpaH I
Bcl I	5 ' - T | G A T C   A - 3 '   3 ' - A   C T A G | T - 5 '	5'-GATC			Fba I, Ksp22 I
Bco I	5 ' - C | Y C G R   G - 3 '   3 ' - G   R G C Y | C - 5 '	5'-YCGR	Ava I		Ama87 I, BsiHKC I, BsoB I, Eco88 I, Nsp III	BmeT110 I
Bcu I	5 ' - A | C T A G   T - 3 '   3 ' - T   G A T C | A - 5 '	5'-CTAG	Spe I		Ahl I
Bfr I	5 ' - C | T T A A   G - 3 '   3 ' - G   A A T T | C - 5 '	5'-TTAA	MspC I		Afl II, BspT I, Bst98 I, Vha464 I
BfuC I	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II	BstKT I
Bgl I	5 ' - G C C N   N N N | N G G C - 3 '   3 ' - C G G N | N N N   N C C G - 5 '	NNN-3'
Bgl II	5 ' - A | G A T C   T - 3 '   3 ' - T   C T A G | A - 5 '	5'-GATC
Bme18 I	5 ' - G | G W C   C - 3 '   3 ' - C   C W G | G - 5 '	5'-GWC	Sin I		Ava II, Afl I, Eco47 I, VpaK11B I
BmeT110 I	5 ' - C Y | C G R   G - 3 '   3 ' - G R   G C | Y C - 5 '	5'-CG		Ava I 5 ' - C | Y C G R   G - 3 '   3 ' - G   R G C Y | C - 5 '		Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp III
Bmt I	5 ' - G   C T A G | C - 3 '   3 ' - C | G A T C   G - 5 '	CTAG-3'		Nhe I 5 ' - G | C T A G   C - 3 '   3 ' - C   G A T C | G - 5 '		AsuNH I
Bpu14 I	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
BpvU I	5 ' - C G   A T | C G - 3 '   3 ' - G C | T A   G C - 5 '	AT-3'	Pvu I		BspC I, Mvr I, Ple19 I
Bsa29 I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bse118 I	5 ' - R C | C G   G Y - 3 '   3 ' - Y G   G C | C R - 5 '	5'-CG	BssA I		BsrF I, Cfr10 I
BseA I	5 ' - T | C C G G   A - 3 '   3 ' - A   G G C C | T - 5 '	5'-CCGG			Acc III, Bsp13 I, BspE I, Kpn2 I, Mro I
BseB I	5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	5'-W			BstN I, BstO I, Bst2U I, Mva I	Ajn I, EcoR II, Psp6 I, PspG I
BseC I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG			Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
BseG I	5 ' - G G A T G   N N | - 3 '   3 ' - C C T A C | N N   - 5 '	NN-3'		Fok I 5 ' - G G A T G ( N ) 9 | N N N N   - 3 '   3 ' - C C T A C ( N ) 9   N N N N | - 5 '	BstF5 I, BtsC I
BseP I	5 ' - G | C G C G   C - 3 '   3 ' - C   G C G C | G - 5 '	5'-CGCG	BssH II		Pau I
BshF I	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt			BspAN I, BsuR I, Hae III, Pal I, Pho I
BshT I	5 ' - A | C C G G   T - 3 '   3 ' - T   G G C C | A - 5 '	5'-CCGG	CspA I		Age I, AsiA I, PinA I
BsiHKC I	5 ' - C | Y C G R   G - 3 '   3 ' - G   R G C Y | C - 5 '	5'-YCGR	Ava I		Ama87 I, Bco I, BsoB I, Eco88 I, Nsp III	BmeT110 I
BsiS I	5 ' - C | C G   G - 3 '   3 ' - G   G C | C - 5 '	5'-CG			Hap II, Hpa II, Msp I
BsoB I	5 ' - C | Y C G R   G - 3 '   3 ' - G   R G C Y | C - 5 '	5'-YCGR	Ava I		Ama87 I, Bco I, BsiHKC I, Eco88 I, Nsp III	BmeT110 I
Bsp106 I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bsp119 I	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bpu14 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
Bsp120 I	5 ' - G | G G C C   C - 3 '   3 ' - C   C C G G | G - 5 '	5'-GGCC		Apa I 5 ' - G   G G C C | C - 3 '   3 ' - C | C C G G   G - 5 '	PspOM I
Bsp13 I	5 ' - T | C C G G   A - 3 '   3 ' - A   G G C C | T - 5 '	5'-CCGG	BseA I		Acc III, BspE I, Kpn2 I, Mro I
Bsp143 I	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II	BstKT I
Bsp19 I	5 ' - C | C A T G   G - 3 '   3 ' - G   G T A C | C - 5 '	5'-CATG	Nco I
Bsp68 I	5 ' - T C G | C G A - 3 '   3 ' - A G C | G C T - 5 '	blunt	Nru I
BspAN I	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt	BshF I		BsuR I, Hae III, Pal I, Pho I
BspC I	5 ' - C G   A T | C G - 3 '   3 ' - G C | T A   G C - 5 '	AT-3'	Pvu I		BpvU I, Mvr I, Ple19 I
BspD I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, Bsp106 I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
BspE I	5 ' - T | C C G G   A - 3 '   3 ' - A   G G C C | T - 5 '	5'-CCGG	BseA I		Acc III, Bsp13 I, Kpn2 I, Mro I
BspT I	5 ' - C | T T A A   G - 3 '   3 ' - G   A A T T | C - 5 '	5'-TTAA	MspC I		Afl II, Bfr I, Bst98 I, Vha464 I
BspT104 I	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bpu14 I, Bsp119 I, BstB I, Csp45 I, Nsp V, Sfu I
BspX I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, Bsp106 I, BspD I, Bsu15 I, BsuTU I, Cla I, Zho I
BsrF I	5 ' - R C | C G   G Y - 3 '   3 ' - Y G   G C | C R - 5 '	5'-CG	Bss A I		Bse118 I, Cfr10 I
BssA I	5 ' - R | C C G G   Y - 3 '   3 ' - Y   G G C C | R - 5 '	5'-CG			Bse118 I, BsrF I, Cfr10 I
BssH II	5 ' - G | C G C G   C - 3 '   3 ' - C   G C G C | G - 5 '	5'-TCGA	Sla I		PaeR7 I, Sfr274 I, Tli I, Xho I
BssT1 I	5 ' - C | C W W G   G - 3 '   3 ' - G   G W W C | C - 5 '	5'-CWWG	Sty I		Eco130 I, EcoT14 I, Erh I
Bst2U I	5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	5'-W	Bse B I		BstN I, BstO I, Mva I	Ajn I, EcoR II, Psp6 I, PspG I
Bst98 I	5 ' - C | T T A A   G - 3 '   3 ' - G   A A T T | C - 5 '	5'-TTAA	MspC I		Afl II, Bfr I, BspT I, Vha464 I
BstB I	5 ' - T T | C G   A A - 3 '   3 ' - A A   G C | T T - 5 '	5'-CG	Asu II		Bpu14 I, Bsp119 I, BspT104 I, Csp45 I, Nsp V ,Sfu I
BstE II	5 ' - G | G T N A C   C - 3 '   3 ' - C   C A N T G | G - 5 '	5'-GTNAC			BstP I, Eco91 I, EcoO65 I, PspE I
BstEN II	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, Bsp143 I, BstMB I, Dpn II, Kzo9 I, Nde II	BstKT I
BstF5 I	5 ' - G G A T G   N N | - 3 '   3 ' - C C T A C | N N   - 5 '	NN-3'		Fok I 5 ' - G G A T G ( N ) 9 | N N N N   - 3 '   3 ' - C C T A C ( N ) 9   N N N N | - 5 '	BstG I, BtsC I
BstKT I	5 ' - G   A T | C - 3 '   3 ' - C | T A   G - 5 '	AT-3'		Mbo I Sau3A I 5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '		BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II
				Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '
BstMA I	5 ' - C   T G C A | G - 3 '   3 ' - G | A C G T   C - 5 '	TGCA-3'	Pst I
BstMB I	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Mbo I Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, Bsp143 I, BstEN II, Dpn II, Kzo9 I, Nde II	BstKT I
BstN I	5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	5'-W	BseB I		BstO I, Bst2U I, Mva I	Ajn I, EcoR II, Psp6 I, PspG I
BstO I	5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	5'-W	BseB I		BstN I, Bst2U I, Mva I	Ajn I, EcoR II, Psp6 I, PspG I
BstP I	5 ' - G | G T N A C   C - 3 '   3 ' - C   C A N T G | G - 5 '	5'-GTNAC	BstE II		Eco91 I, EcoO65 I, PspE I
BstSN I	5 ' - T A C | G T A - 3 '   3 ' - A T G | C A T - 5 '	blunt	SnaB I		Eco105 I
BstX I	5 ' - C C A N   N N N N | N T G G - 3 '   3 ' - G G T N | N N N N   N A C C - 5 '	NNNN-3'
Bsu15 I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, BsuTU I, Cla I, Zho I
BsuR I	5 ' - G G | C C - 3 '   3 ' - C C | G G - 5 '	blunt	BshF I		BspAN I, Hae III, Pal I, Pho I
BsuTU I	5 ' - A T | C G   A T - 3 '   3 ' - T A   G C | T A - 5 '	5'-CG	BseC I		Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, Cla I, Zho I
BtsC I	5 ' - G G A T G   N N | - 3 '   3 ' - C C T A C | N N   - 5 '	NN-3'		Fok I 5 ' - G G A T G ( N ) 9 | N N N N   - 3 '   3 ' - C C T A C ( N ) 9   N N N N | - 5 '	BseG I, BstF5 I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


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