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EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
BamH I
5
'
-
G
|
G
A
T
C
C
-
3
'

3
'
-
C
C
T
A
G
|
G
-
5
'
5'-GATC



Ban II
5
'
-
G
R
G
C
Y
|
C
-
3
'

3
'
-
C
|
Y
C
G
R
G
-
5
'
RGCY-3'

Eco24 I, EcoT38 I, FriO I
Ban III
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bbe I
5
'
-
G
G
C
G
C
|
C
-
3
'

3
'
-
C
|
C
G
C
G
G
-
5
'
GCGC-3'
Nar I

5
'
-
G
G
|
C
G
C
C
-
3
'

3
'
-
C
C
G
C
|
G
G
-
5
'

Din I, Ege I, Ehe I, Kas I, Mly113 I, Sfo I
Bbu I
5
'
-
G
C
A
T
G
|
C
-
3
'

3
'
-
C
|
G
T
A
C
G
-
5
'
CATG-3'Sph I
Pae I, SpaH I
Bcl I
5
'
-
T
|
G
A
T
C
A
-
3
'

3
'
-
A
C
T
A
G
|
T
-
5
'
5'-GATC

Fba I, Ksp22 I
Bco I
5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'
5'-YCGRAva I
Ama87 I, BsiHKC I, BsoB I, Eco88 I, Nsp IIIBmeT110 I
Bcu I
5
'
-
A
|
C
T
A
G
T
-
3
'

3
'
-
T
G
A
T
C
|
A
-
5
'
5'-CTAGSpe I
Ahl I
Bfr I
5
'
-
C
|
T
T
A
A
G
-
3
'

3
'
-
G
A
A
T
T
|
C
-
5
'
5'-TTAAMspC I
Afl II, BspT I, Bst98 I, Vha464 I
BfuC I
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde IIBstKT I
Bgl I
5
'
-
G
C
C
N
N
N
N
|
N
G
G
C
-
3
'

3
'
-
C
G
G
N
|
N
N
N
N
C
C
G
-
5
'
NNN-3'



Bgl II
5
'
-
A
|
G
A
T
C
T
-
3
'

3
'
-
T
C
T
A
G
|
A
-
5
'
5'-GATC



Bme18 I
5
'
-
G
|
G
W
C
C
-
3
'

3
'
-
C
C
W
G
|
G
-
5
'
5'-GWCAva II
Afl I, Eco47 I, Sin I, VpaK11B I
BmeT110 I
5
'
-
C
Y
|
C
G
R
G
-
3
'

3
'
-
G
R
G
C
|
Y
C
-
5
'
5'-CG
Ava I

5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'

Ama87 I, Bco I, BsiHKC I, BsoB I, Eco88 I, Nsp III
Bmt I
5
'
-
G
C
T
A
G
|
C
-
3
'

3
'
-
C
|
G
A
T
C
G
-
5
'
CTAG-3'
Nhe I

5
'
-
G
|
C
T
A
G
C
-
3
'

3
'
-
C
G
A
T
C
|
G
-
5
'

AsuNH I
Bpu14 I
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CGAsu II
Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
BpvU I
5
'
-
C
G
A
T
|
C
G
-
3
'

3
'
-
G
C
|
T
A
G
C
-
5
'
AT-3'Pvu I
BspC I, Mvr I, Ple19 I
Bsa29 I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bse118 I
5
'
-
R
C
|
C
G
G
Y
-
3
'

3
'
-
Y
G
G
C
|
C
R
-
5
'
5'-CGBssA I
BsrF I, Cfr10 I
BseA I
5
'
-
T
|
C
C
G
G
A
-
3
'

3
'
-
A
G
G
C
C
|
T
-
5
'
5'-CCGG

Acc III, Bsp13 I, BspE I, Kpn2 I, Mro I
BseB I
5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
5'-W

BstN I, BstO I, Bst2U I, Mva IAjn I, EcoR II, Psp6 I, PspG I
BseC I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CG

Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
BseG I
5
'
-
G
G
A
T
G
N
N
|
-
3
'

3
'
-
C
C
T
A
C
|
N
N
-
5
'
NN-3'
Fok I

5
'
-
G
G
A
T
G
(
N
)
9
|
N
N
N
N
-
3
'

3
'
-
C
C
T
A
C
(
N
)
9
N
N
N
N
|
-
5
'
BstF5 I, BtsC I
BseP I
5
'
-
G
|
C
G
C
G
C
-
3
'

3
'
-
C
G
C
G
C
|
G
-
5
'
5'-CGCGBssH II
Pau I
BshF I
5
'
-
G
G
|
C
C
-
3
'

3
'
-
C
C
|
G
G
-
5
'
blunt

BspAN I, BsuR I, Hae III, Pal I, Pho I
BshT I
5
'
-
A
|
C
C
G
G
T
-
3
'

3
'
-
T
G
G
C
C
|
A
-
5
'
5'-CCGGCspA I
Age I, AsiA I, PinA I
BsiHKC I
5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'
5'-YCGRAva I
Ama87 I, Bco I, BsoB I, Eco88 I, Nsp IIIBmeT110 I
BsiS I
5
'
-
C
|
C
G
G
-
3
'

3
'
-
G
G
C
|
C
-
5
'
5'-CGHpa II
Hap II, Msp I
BsoB I
5
'
-
C
|
Y
C
G
R
G
-
3
'

3
'
-
G
R
G
C
Y
|
C
-
5
'
5'-YCGRAva I
Ama87 I, Bco I, BsiHKC I, Eco88 I, Nsp IIIBmeT110 I
Bsp106 I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
Bsp119 I
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, BspT104 I, BstB I, Csp45 I, Nsp V, Sfu I
Bsp120 I
5
'
-
G
|
G
G
C
C
C
-
3
'

3
'
-
C
C
C
G
G
|
G
-
5
'
5'-GGCC
Apa I

5
'
-
G
G
G
C
C
|
C
-
3
'

3
'
-
C
|
C
C
G
G
G
-
5
'
PspOM I
Bsp13 I
5
'
-
T
|
C
C
G
G
A
-
3
'

3
'
-
A
G
G
C
C
|
T
-
5
'
5'-CCGGBseA I
Acc III, BspE I, Kpn2 I, Mro I
Bsp143 I
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BfuC I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde IIBstKT I
Bsp19 I
5
'
-
C
|
C
A
T
G
G
-
3
'

3
'
-
G
G
T
A
C
|
C
-
5
'
5'-CATGNco I


Bsp68 I
5
'
-
T
C
G
|
C
G
A
-
3
'

3
'
-
A
G
C
|
G
C
T
-
5
'
bluntNru I


BspAN I
5
'
-
G
G
|
C
C
-
3
'

3
'
-
C
C
|
G
G
-
5
'
bluntBshF I
BsuR I, Hae III, Pal I, Pho I
BspC I
5
'
-
C
G
A
T
|
C
G
-
3
'

3
'
-
G
C
|
T
A
G
C
-
5
'
AT-3'Pvu I
BpvU I, Mvr I, Ple19 I
BspD I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, Bsp106 I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
BspE I
5
'
-
T
|
C
C
G
G
A
-
3
'

3
'
-
A
G
G
C
C
|
T
-
5
'
5'-CCGGBseA I
Acc III, Bsp13 I, Kpn2 I, Mro I
BspT I
5
'
-
C
|
T
T
A
A
G
-
3
'

3
'
-
G
A
A
T
T
|
C
-
5
'
5'-TTAAMspC I
Afl II, Bfr I, Bst98 I, Vha464 I
BspT104 I
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, Bsp119 I, BstB I, Csp45 I, Nsp V, Sfu I
BspX I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, Bsp106 I, BspD I, Bsu15 I, BsuTU I, Cla I, Zho I
BsrF I
5
'
-
R
C
|
C
G
G
Y
-
3
'

3
'
-
Y
G
G
C
|
C
R
-
5
'
5'-CGBss A I
Bse118 I, Cfr10 I
BssA I
5
'
-
R
|
C
C
G
G
Y
-
3
'

3
'
-
Y
G
G
C
C
|
R
-
5
'
5'-CG

Bse118 I, BsrF I, Cfr10 I
BssH II
5
'
-
G
|
C
G
C
G
C
-
3
'

3
'
-
C
G
C
G
C
|
G
-
5
'
5'-TCGASla I
PaeR7 I, Sfr274 I, Tli I, Xho I
BssT1 I
5
'
-
C
|
C
W
W
G
G
-
3
'

3
'
-
G
G
W
W
C
|
C
-
5
'
5'-CWWGSty I
Eco130 I, EcoT14 I, Erh I
Bst2U I
5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
5'-WBse B I
BstN I, BstO I, Mva IAjn I, EcoR II, Psp6 I, PspG I
Bst98 I
5
'
-
C
|
T
T
A
A
G
-
3
'

3
'
-
G
A
A
T
T
|
C
-
5
'
5'-TTAAMspC I
Afl II, Bfr I, BspT I, Vha464 I
BstB I
5
'
-
T
T
|
C
G
A
A
-
3
'

3
'
-
A
A
G
C
|
T
T
-
5
'
5'-CGAsu II
Bpu14 I, Bsp119 I, BspT104 I, Csp45 I, Nsp V ,Sfu I
BstE II
5
'
-
G
|
G
T
N
A
C
C
-
3
'

3
'
-
C
C
A
N
T
G
|
G
-
5
'
5'-GTNAC

BstP I, Eco91 I, EcoO65 I, PspE I
BstEN II
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstMB I, Dpn II, Kzo9 I, Nde IIBstKT I
BstF5 I
5
'
-
G
G
A
T
G
N
N
|
-
3
'

3
'
-
C
C
T
A
C
|
N
N
-
5
'
NN-3'
Fok I

5
'
-
G
G
A
T
G
(
N
)
9
|
N
N
N
N
-
3
'

3
'
-
C
C
T
A
C
(
N
)
9
N
N
N
N
|
-
5
'
BstG I, BtsC I
BstKT I
5
'
-
G
A
T
|
C
-
3
'

3
'
-
C
|
T
A
G
-
5
'
AT-3'
Mbo I Sau3A I

5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'

BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II

Dpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BstMA I
5
'
-
C
T
G
C
A
|
G
-
3
'

3
'
-
G
|
A
C
G
T
C
-
5
'
TGCA-3'Pst I


BstMB I
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCMbo I

Sau3A I
Dpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstEN II, Dpn II, Kzo9 I, Nde IIBstKT I
BstN I
5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
5'-WBseB I
BstO I, Bst2U I, Mva IAjn I, EcoR II, Psp6 I, PspG I
BstO I
5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
5'-WBseB I
BstN I, Bst2U I, Mva IAjn I, EcoR II, Psp6 I, PspG I
BstP I
5
'
-
G
|
G
T
N
A
C
C
-
3
'

3
'
-
C
C
A
N
T
G
|
G
-
5
'
5'-GTNACBstE II
Eco91 I, EcoO65 I, PspE I
BstSN I
5
'
-
T
A
C
|
G
T
A
-
3
'

3
'
-
A
T
G
|
C
A
T
-
5
'
bluntSnaB I
Eco105 I
BstX I
5
'
-
C
C
A
N
N
N
N
N
|
N
T
G
G
-
3
'

3
'
-
G
G
T
N
|
N
N
N
N
N
A
C
C
-
5
'
NNNN-3'



Bsu15 I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, BsuTU I, Cla I, Zho I
BsuR I
5
'
-
G
G
|
C
C
-
3
'

3
'
-
C
C
|
G
G
-
5
'
bluntBshF I
BspAN I, Hae III, Pal I, Pho I
BsuTU I
5
'
-
A
T
|
C
G
A
T
-
3
'

3
'
-
T
A
G
C
|
T
A
-
5
'
5'-CGBseC I
Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, Cla I, Zho I
BtsC I
5
'
-
G
G
A
T
G
N
N
|
-
3
'

3
'
-
C
C
T
A
C
|
N
N
-
5
'
NN-3'
Fok I

5
'
-
G
G
A
T
G
(
N
)
9
|
N
N
N
N
-
3
'

3
'
-
C
C
T
A
C
(
N
)
9
N
N
N
N
|
-
5
'
BseG I, BstF5 I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

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