A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z

Enzyme	Cleavage Site	Overhang	Iso-/Neoschizomer at JBS		Other Iso-/Neoschizomers
			Iso	Neo (Cleavage Site)	Iso	Neo
Mbo I	5 ' - | G A T C   - 3 '   3 ' -   C T A G | - 5 '	5'-GATC	Sau3A I	Dpn I 5 ' - G A | T C - 3 '   3 ' - C T | A G - 5 '	BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II	BstKT I
Mbo II	5 ' - G A A G A N N N N N N N   N | - 3 '   3 ' - C T T C T N N N N N N N | N   - 5 '	N-3'
Mlu I	5 ' - A | C G C G   T - 3 '   3 ' - T   G C G C | A - 5 '	5'-CGCG
Mly113 I	5 ' - G G | C G   C C - 3 '   3 ' - C C   G C | G G - 5 '	5'-CG	Nar I			Bbe I, Din I, Ege I, Ehe I, Sfo I, Kas I
Mnl I	5 ' - C C T C N N N N N N   N | - 3 '   3 ' - G G A G N N N N N N | N   - 5 '	N-3'
Mro I	5 ' - T | C C G G   A - 3 '   3 ' - A   G G C C | T - 5 '	5'-CCGG	BseA I		Acc III, Bsp13 I, BspE I, Kpn2 I
MroN I	5 ' - G | C C G G   C - 3 '   3 ' - C   G G C C | G - 5 '	5'-CCGG		Nae I 5 ' - G C C | G G C - 3 '   3 ' - C G G | C C G - 5 '	NgoM IV	Pdi I
Msp I	5 ' - C | C G   G - 3 '   3 ' - G   G C | C - 5 '	5'-CG	BsiS I		Hap II, Hpa II
MspC I	5 ' - C | T T A A   G - 3 '   3 ' - G   A A T T | C - 5 '	5'-TTAA			Afl II, Bfr I, BspT I, Bst98 I, Vha464 I
Mva I	5 ' - C C | W   G G - 3 '   3 ' - G G   W | C C - 5 '	5'-W	BseB I		BstN I, BstO I, Bst2U I	Ajn I, EcoR II, Psp6 I, PspG I
Mvr I	5 ' - C G   A T | C G - 3 '   3 ' - G C | T A   G C - 5 '	AT-3'	Pvu I		BpvU I, BspC I, Ple19 I

IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T


Please contact enzymes@jenabioscience.com for more information.