A  |  B  |  C  |  D  |  E  |  F  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  V  |  X  |  Z


 
EnzymeCleavage SiteOverhangIso-/Neoschizomer at JBSOther Iso-/Neoschizomers
IsoNeo
(Cleavage Site)
IsoNeo
Mbo I
5
'
-
|
G
A
T
C
-
3
'

3
'
-
C
T
A
G
|
-
5
'
5'-GATCSau3A IDpn I

5
'
-
G
A
|
T
C
-
3
'

3
'
-
C
T
|
A
G
-
5
'
BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde IIBstKT I
Mbo II
5
'
-
G
A
A
G
A
N
N
N
N
N
N
N
N
|
-
3
'

3
'
-
C
T
T
C
T
N
N
N
N
N
N
N
|
N
-
5
'
N-3'



Mlu I
5
'
-
A
|
C
G
C
G
T
-
3
'

3
'
-
T
G
C
G
C
|
A
-
5
'
5'-CGCG



Mly113 I
5
'
-
G
G
|
C
G
C
C
-
3
'

3
'
-
C
C
G
C
|
G
G
-
5
'
5'-CGNar I

Bbe I, Din I, Ege I, Ehe I, Sfo I, Kas I
Mnl I
5
'
-
C
C
T
C
N
N
N
N
N
N
N
|
-
3
'

3
'
-
G
G
A
G
N
N
N
N
N
N
|
N
-
5
'
N-3'



Mro I
5
'
-
T
|
C
C
G
G
A
-
3
'

3
'
-
A
G
G
C
C
|
T
-
5
'
5'-CCGGBseA I
Acc III, Bsp13 I, BspE I, Kpn2 I
MroN I
5
'
-
G
|
C
C
G
G
C
-
3
'

3
'
-
C
G
G
C
C
|
G
-
5
'
5'-CCGG
Nae I

5
'
-
G
C
C
|
G
G
C
-
3
'

3
'
-
C
G
G
|
C
C
G
-
5
'
NgoM IVPdi I
Msp I
5
'
-
C
|
C
G
G
-
3
'

3
'
-
G
G
C
|
C
-
5
'
5'-CGHpa II
BsiS I, Hap II
MspC I
5
'
-
C
|
T
T
A
A
G
-
3
'

3
'
-
G
A
A
T
T
|
C
-
5
'
5'-TTAA

Afl II, Bfr I, BspT I, Bst98 I, Vha464 I
Mva I
5
'
-
C
C
|
W
G
G
-
3
'

3
'
-
G
G
W
|
C
C
-
5
'
5'-WBseB I
BstN I, BstO I, Bst2U IAjn I, EcoR II, Psp6 I, PspG I
Mvr I
5
'
-
C
G
A
T
|
C
G
-
3
'

3
'
-
G
C
|
T
A
G
C
-
5
'
AT-3'Pvu I
BpvU I, BspC I, Ple19 I

 
IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T

 

Please contact enzymes@jenabioscience.com for more information.