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A | B | C | D | E | F | H | K | M | N | P | R | S | T | V | X | Z
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| Enzyme | Cleavage Site | Overhang | Iso-/Neoschizomer at JBS | Other Iso-/Neoschizomers | | Iso | Neo
(Cleavage Site) | Iso | Neo | | Mbo I | 5 ' - | G A T C - 3 ' 3 ' - C T A G | - 5 ' | 5'-GATC | Sau3A I | Dpn I
| BfuCI, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 I, Nde II | BstKTI | | Mro I | 5 ' - T | C C G G A - 3 ' 3 ' - A G G C C | T - 5 ' | 5'-CCGG | BseA I |
| Acc III, Bsp13 I, BspE I, Kpn2 I |
| | MroN I | 5 ' - G | C C G G C - 3 ' 3 ' - C G G C C | G - 5 ' | 5'-CCGG |
| Nae I
5 ' - G C C | G G C - 3 ' 3 ' - C G G | C C G - 5 ' | NgoM IV | Pdi I | | Msp I | 5 ' - C | C G G - 3 ' 3 ' - G G C | C - 5 ' | 5'-CG | BsiS I |
| Hap II, Hpa II |
| | MspC I | 5 ' - C | T T A A G - 3 ' 3 ' - G A A T T | C - 5 ' | 5'-TTAA |
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| Afl II, Bfr I, BspT I, Bst98 I, Vha464 I |
| | Mva I | 5 ' - C C | W G G - 3 ' 3 ' - G G W | C C - 5 ' | 5'-W | BseB I |
| BstN I, BstO I, Bst2U I | Ajn I, EcoR II, Psp6 I, PspG I |
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IUB-Codes: M=A or C, R=A or G, W=A or T, S=G or C, Y=C or T, K=G or T, V=A or G or C, H=A or C or T, D=A or G or T, B=G or T or C, N=A or G or C or T
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Please contact enzymes@jenabioscience.com for more information.
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