Solubility and functionality of expressed proteins

Incorrect folding and insufficient solubility - resulting in compromised biological activity - are the major shortcomings of prokaryotic expression systems. Due to LEXSY’s fully eukaryotic protein synthesis/folding/modification machinery most proteins of higher organisms expressed in LEXSY are correctly folded and processed and therefore, are obtained in a fully functional state.

Selected examples of LEXSY-expressed proteins with full biologic activity:

Protein	Localisat.	Origin	Reference	Downloads
Erythropoietin	sec	human	Breitling et al. 2002
Surface Antigen 1& 2	sec	Toxoplasma gondii	Ebert et al. 2007 not publ.
Proprotein Convertase 4	sec	rat	Basak et al. 2008
Laminin-332	sec	human	Phan et al. 2009
Cu/Zn superoxide dismutase	cyt	human	Gazdag et al. 2010
Tissue Plasminogen Activator	sec	human	Hemayatkar et al. 2010
N-Acetyl Serotonin Methyl Transferase (ASMT)	cyt	human	Ben-Abdallah et al. 2010
Hydroxynitrile Lyase (MeHNL)	cyt	cassava plant	Dadashipour et al. 2011
Coagulation factor VII	cyt	human	Mirzaahmadi et al. 2011

For a short summary of expression data obtained, please, refer to the LEXSY Brochure page 18