Solubility and functionality of expressed proteins

Incorrect folding and insufficient solubility - resulting in compromised biological activity - are the major shortcomings of prokaryotic expression systems. Due to LEXSYs fully eukaryotic protein synthesis/folding/modification machinery most proteins of higher organisms expressed in LEXSY are correctly folded and processed and therefore, are obtained in a fully functional state.


Selected examples of LEXSY-expressed proteins with full biologic activity:
 
ProteinLocalisat.OriginReferenceDownloads
ErythropoietinsechumanBreitling et al. 2002PDF download
Surface Antigen 1& 2secToxoplasma gondiiEbert et al. 2007 not publ.
Proprotein Convertase 4 secratBasak et al. 2008PDF download
Laminin-332sechumanPhan et al. 2009PDF download
Cu/Zn superoxide dismutasecythumanGazdag et al. 2010PDF download
Tissue Plasminogen ActivatorsechumanHemayatkar et al. 2010PDF download
N-Acetyl Serotonin Methyl Transferase (ASMT)cythumanBen-Abdallah et al. 2010PDF download
Hydroxynitrile Lyase (MeHNL)cytcassava plantDadashipour et al. 2011PDF download
Coagulation factor VIIcythumanMirzaahmadi et al. 2011PDF download

 
For a short summary of expression data obtained, please, refer to the LEXSY Brochure page 18