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Oligo sequence:
OD value:
 
Length: Bases
GC content: %
Molecular Weight (free acid): g/mol
Molecular Weight (ammonia salt): g/mol
Melting Temperature (Tm)1 °C
Melting Temperature (Tm)2 °C
Concentration: µg/ml    µM

1 The classical formula Tm = 2(A + T) + 4(G + C) is used for melting temperature calculation, according to Wallace rule for short oligonuceotides [1]. Normally used for short oligonucleotides <= 20 bases.

2 The calculation is based on the Nearest-Neighbor formula with [salt] = 50 mM and single strand concentration = 250 pM [2]. Normally used for oligonucleotides > 20 bases.

[1] Wallace et al. (1979) Nucleic Acid Res. 6: 3543.
[2] Breslauer et al. (1986) Proc. Natl. Acad. Sci. USA 83: 3746.