Immobilized or purified antibodies, also termed immunoglobulins, are of major importance both for immunochemical techniques in basic research (e.g. Immunoprecipitation) and for diagnostic applications.

One of the most successful immobilization and purification technique is based on the high affinity and binding specificity of Protein A, a surface protein of Staphylococcus aureus, towards the Fc region of a broad range of mammalian immunoglobulins (Ig).

Immunoglobulins from different species and different isotypes within a species (IgA, IgG, IgM, IgE, IgD) differ in their affinity to Protein A [1,2].

Refer to Protein A affinity towards various species and IgG isotypes for a comprehensive overview.

Protein A Agarose

High-performance 4 % cross-linked agarose suitable for the purification of a broad range of immunoglobulins from a variety of mammalian species and different isotypes

Selected References:

[1] Harlow and Lane (eds.) (1988) Antibodies. A Laboratory Manual. Cold Spring Harbor Laboratory, N.Y., 617-618.

[2] Richmann et al. (1982) The binding of staphylococcaI protein A by the sera of different animal species. J. Immunol. 128:2300.