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HIT OPTIMIZATION
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Strategies to optimize initial crystallization hits:
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Chemical Environment
Kits and screens to optimize the chemical composition of your crystallization reagents, i.e. buffer pH, precipitant and additive compostion.
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Input Protein
Get your protein ready for crystallization! Consider to optimize the solubilization buffer, alter the protein surface or modify the sequence by truncation or mutagenesis. Or try a new mode of production.
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Thermodynamics / Kinetics
Do not discard bad diffracting crystals! They can be very useful, too. Try to improve diffraction by varying the solvent content or use your initial crystals for seeding.
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