HIT OPTIMIZATION


Strategies to optimize initial crystallization hits:

 

Thermodynamics/KineticsInput ProteinChemical EnvironmentOptimization - Flow Chart

 



Optimization - Chemical Environment

Chemical Environment

Kits and screens to optimize the chemical composition of your crystallization reagents, i.e. buffer pH, precipitant and additive compostion.

 



Optimization - Input Protein

Input Protein

Get your protein ready for crystallization! Consider to optimize the solubilization buffer, alter the protein surface or modify the sequence by truncation or mutagenesis. Or try a new mode of production.

 



Optimization - Thermodynamics / Kinetics

Thermodynamics / Kinetics

Do not discard bad diffracting crystals! They can be very useful, too. Try to improve diffraction by varying the solvent content or use your initial crystals for seeding.