Labeling of proteins with fluorescent reporter dyes is a prominent method for their characterization with respect to their structure, folding, and interaction with other proteins both in biochemical and in cellular investigations. The labeling kits presented here are designed for either site-specific labeling or non site-specific labeling employing
  • exceptionally convenient and mild labeling conditions
  • multi-color labeling for fluorescence resonance energy transfer (FRET) studies
  • thoroughly selected fluorophores with high photostability suitable for a broad range of established and novel techniques


    Site-specific labeling of C- and/or N-terminus

    Universal method for labeling of any protein at its C-terminus and/or at its N-terminal cystein – suitable for One-Pot Dual Color Labeling. C-terminal modification is based on fast and convenient oxyamine-ketone ligation. For N-terminal modification, an N-terminal cysteine undergoes a native chemical ligation with a fluorophore-thioester.

    Amine and Thiol Protein Labeling

    Universal method for labeling of any protein‘s free amino groups (N-terminus, lysines) or its thiol groups (cysteins).
    Outstanding photostability and quantum yield of thoroughly selected dyes combined with straightforward labeling protocols.

    Luminescent Protein Labeling

    Universal method for labeling of any protein with luminescent ruthenium complexes employing exceptionally long lifetimes of luminescence (up to 1 µs) and large stokes shifts (more than 100 nm). Resulting high signal to noise ratios allow application in anisotropy studies and fluorescence lifetime imaging.