Properties of Fluorescent Dyes

From the ever increasing number of commercially available fluorescent dyes we have thoroughly selected those dyes that:

• cover the whole UV/VIS spectrum with all colors available
• fit the common commercial light sources and filter systems
• have superiour properties with primary focus on
  
  • signal intensity
  • high solubility in water
  • photostability (minimal photobleaching)
  • low molecular weight resulting in minimal steric hindrance

Fluorescent Color	Dye	MW (free acid)	λabs	λem	εmax	Isomers	Notes/Advantages
		[g/mol]	[nm]	[nm]	[cm-1*M-1]
Near-IR	DY-776	835	771	801	120,000	pure	Soluble in water, negatively charged, spectrum similar to LICOR IRD 800RS
Near-IR	DY-751	815	751	779	220,000	pure
Near-IR	ATTO 740	568	740	764	120,000	pure
Near-IR	ATTO 725	516	725	752	120,000	pure
Red	ATTO 700	575	700	719	120,000	pure	Substitute for Cy5.5
Red	ATTO 680	631	680	700	125,000	pure	Substitute for Cy5.5; spectroscopic properties similar to LICOR IRD 700
Red	ATTO 665	623	663	684	160,000	pure	Suitable for STED applications
Red	ATTO 655	634	663	684	125,000	pure	Substitute for Cy5, but better since no triplet behaviour
Red	OYSTER-656	737	656	674	200,000	pure	Higher photostability compared to Cy5
Red	Cy5	658	649	670	250,000	pure
Red	ATTO 647N	746	644	669	150,000	two	Substitute for Alexa Fluor 647 and Cy5, base stable
Red	ATTO 633	652	629	657	130,000	pure	Emission maximum of He-Ne laser, base stable, less hydrophilic
Red	ATTO Rho14	784	625	646	120,000	pure
Red	ATTO 620	612	619	643	120,000	pure	Substitute for LightCycler Red 640, base stable
Red	DY-480XL	515	500	630	50,000	pure	Large Stokes Shift of 130 nm
Red	ATTO 594	1137	601	627	120,000	pure	Substitute for Alexa-594, more hydrophilic than ATTO 590
Orange	ATTO Rho13	646	600	625	125,000	pure
Orange	ATTO 590	691	594	624	120,000	two	Substitute for Texas Red / Alexa-594
Orange	ATTO Rho101	590	586	610	120,000	pure
Orange	Texas Red	725	588	609	80,000	pure
Orange	ATTO Thio12	503	579	609	110,000	pure
Orange	ATTO Rho12	650	576	601	120,000	pure
Orange	6-ROX	535	575	600	82,000	pure
Orange	ATTO Rho11	567	571	595	120,000	pure
Orange	ATTO 565	611	563	592	120,000	two	Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
Yellow	ATTO 550	694	554	576	120,000	three (60% : 22% : 17%)	Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
Yellow	5/6-TAMRA	430	545	575	90,000	two
Yellow	Cy3	632	550	570	150,000	pure
Yellow-Green	ATTO Rho6G	514	535	560	115,000	pure
Yellow-Green	DY-485XL	503	485	560	50,000	pure	Large Stokes Shift of 75 nm
Yellow-Green	ATTO 532	765	532	553	115,000	pure	Substitute for Alexa Fluor 532 / Bodipy 530/550
Yellow-Green	6-JOE	504	520	548	75,000	pure	Substitute for VIC / HEX
Green	ATTO 495	452	495	527	80,000	pure
Green	ATTO 488	804	501	523	90,000	pure	Substitute for FITC / Alexa Fluor 488 / Cy2, identical to Alexa, much more photostable than Cy2 or FITC
Green	6-FAM	376	492	517	83,000	pure
Green	5-FAM	376	492	517	83,000	pure
Blue-Green	ATTO 465	396	453	508	75,000	pure	Substitute for Cyan 500
Blue-Green	ATTO 425	401	436	484	45,000	pure
Blue	ATTO 390	343	390	479	24,000	pure
Blue	MANT	151	335	440	2,700	pure

Please contact nucleotides@jenabioscience.com with questions or inquir