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Properties of Fluorescent Dyes
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From the ever increasing number of commercially available fluorescent dyes we have thoroughly selected those dyes that:
- cover the whole UV/VIS spectrum with all colors available
- fit the common commercial light sources and filter systems
- have superiour properties with primary focus on
- signal intensity
- high solubility in water
- photostability (minimal photobleaching)
- low molecular weight resulting in minimal steric hindrance
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| Fluorescent Color | Dye | MW (free acid) | λabs | λem | εmax | Isomers | Notes/Advantages |
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| [g/mol] | [nm] | [nm] | [cm-1*M-1] |
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| | Near-IR | DY-751 | 815 | 751 | 779 | 220,000 | pure |
| | Near-IR | ATTO 740 | 568 | 740 | 764 | 120,000 | pure | Alternative dye for LICOR applications | | Near-IR | ATTO 725 | 516 | 725 | 752 | 120,000 | pure | Alternative dye for LICOR applications | | Red | ATTO 700 | 575 | 700 | 719 | 120,000 | pure | Substitute for Cy5.5 | | Red | ATTO 680 | 631 | 680 | 700 | 125,000 | pure | Substitute for Cy5.5 | | Red | ATTO 655 | 634 | 663 | 684 | 125,000 | pure | Substitute for Cy5, but better since no triplet behaviour | | Red | OYSTER-656 | 800 | 656 | 674 | 200,000 | pure | Higher photostability compared to Cy5 | | Red | ATTO 647N | 746 | 644 | 669 | 150,000 | two | Substitute for Alexa Fluor 647 and Cy5, base stable | | Red | ATTO 633 | 652 | 629 | 657 | 130,000 | pure | Emission maximum of He-Ne laser, base stable, less hydrophilic | | Red | ATTO 620 | 612 | 619 | 643 | 120,000 | pure | Substitute for LightCycler Red 640, base stable | | Red | DY-480XL | 515 | 500 | 630 | 50,000 | pure | Large Stokes Shift of 130 nm | | Red | ATTO 594 | 1137 | 601 | 627 | 120,000 | pure | Substitute for Alexa-594, more hydrophilic than ATTO 590 | | Orange | ATTO 590 | 691 | 594 | 624 | 120,000 | pure | Substitute for Texas Red / Alexa-594 | | Orange | Texas Red | 725 | 588 | 609 | 80,000 | pure |
| | Orange | 6-ROX | 535 | 575 | 600 | 82,000 | pure |
| | Orange | ATTO 565 | 611 | 563 | 592 | 120,000 | two | Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability | | Yellow | ATTO 550 | 694 | 554 | 576 | 120,000 | three (60% : 22% : 17%) | Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability | | Yellow | 5/6-TAMRA | 430 | 545 | 575 | 90,000 | two |
| | Yellow-Green | DY-485XL | 503 | 485 | 560 | 50,000 | pure | Large Stokes Shift of 75 nm | | Yellow-Green | ATTO 532 | 765 | 532 | 553 | 115,000 | pure | Substitute for Alexa Fluor 532 / Bodipy 530/550 | | Yellow-Green | 6-JOE | 505 | 520 | 548 | 75,000 | pure | Substitute for VIC / HEX | | Green | ATTO 495 | 452 | 495 | 527 | 80,000 | pure |
| | Green | ATTO 488 | 804 | 501 | 523 | 90,000 | pure | Substitute for FITC / Alexa Fluor 488 / Cy2, identical to Alexa, much more photostable than Cy2 or FITC | | Green | 6-FAM | 376 | 492 | 517 | 83,000 | pure |
| | Green | 5-FAM | 376 | 492 | 517 | 83,000 | pure |
| | Blue-Green | ATTO 465 | 396 | 453 | 508 | 75,000 | pure | Substitute for Cyan 500 | | Blue-Green | ATTO 425 | 401 | 436 | 484 | 45,000 | pure |
| | Blue | ATTO 390 | 343 | 390 | 479 | 24,000 | pure |
| | Blue | MANT | 151 | 335 | 440 | 2,700 | pure |
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Please contact nucleotides@jenabioscience.com with questions or inquir
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