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Properties of Fluorescent Dyes


From the ever increasing number of commercially available fluorescent dyes we have thoroughly selected those dyes that:
  • cover the whole UV/VIS spectrum with all colors available

  • fit the common commercial light sources and filter systems

  • have superiour properties with primary focus on
    • signal intensity
    • high solubility in water
    • photostability (minimal photobleaching)
    • low molecular weight resulting in minimal steric hindrance


Fluorescent ColorDyeMW
(free acid)
λabsλemεmaxIsomersNotes/Advantages


[g/mol][nm][nm][cm-1*M-1]

Near-IRDY-751815751779220,000pure
Near-IRATTO 740568740764120,000pureAlternative dye for LICOR applications
Near-IRATTO 725516725752120,000pureAlternative dye for LICOR applications
RedATTO 700575700719120,000pureSubstitute for Cy5.5
RedATTO 680631680700125,000pureSubstitute for Cy5.5
RedATTO 655634663684125,000pureSubstitute for Cy5, but better since no triplet behaviour
RedOYSTER-656800656674200,000pureHigher photostability compared to Cy5
RedATTO 647N746644669150,000twoSubstitute for Alexa Fluor 647 and Cy5, base stable
RedATTO 633652629657130,000pureEmission maximum of He-Ne laser, base stable, less hydrophilic
RedATTO 620612619643120,000pureSubstitute for LightCycler Red 640, base stable
RedDY-480XL51550063050,000pureLarge Stokes Shift of 130 nm
RedATTO 5941137601627120,000pureSubstitute for Alexa-594, more hydrophilic than ATTO 590
OrangeATTO 590691594624120,000pureSubstitute for Texas Red / Alexa-594
OrangeTexas Red72558860980,000pure
Orange6-ROX53557560082,000pure
OrangeATTO 565611563592120,000twoSubstitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
YellowATTO 550694554576120,000three
(60% : 22% : 17%)
Substitute for Cy3 / Alexa Fluor 546 / Bodipy 558/568, better photostability
Yellow5/6-TAMRA43054557590,000two
Yellow-GreenDY-485XL50348556050,000pureLarge Stokes Shift of 75 nm
Yellow-GreenATTO 532765532553115,000pureSubstitute for Alexa Fluor 532 / Bodipy 530/550
Yellow-Green6-JOE50552054875,000pureSubstitute for VIC / HEX
GreenATTO 49545249552780,000pure
GreenATTO 48880450152390,000pureSubstitute for FITC / Alexa Fluor 488 / Cy2, identical to Alexa, much more photostable than Cy2 or FITC
Green6-FAM37649251783,000pure
Green5-FAM37649251783,000pure
Blue-GreenATTO 46539645350875,000pureSubstitute for Cyan 500
Blue-GreenATTO 42540143648445,000pure
BlueATTO 39034339047924,000pure
BlueMANT1513354402,700pure


Please contact nucleotides@jenabioscience.com with questions or inquir

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