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GSH/Nickel/Cobalt Materials
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The ability to produce recombinant proteins and purify them using affinity chromatography has greatly advanced protein research.
In affinity chromatography, the protein of interest is purified by the ability of its "tag" to bind to a specific ligand that is immobilized by a covalent bond to a chromatographic bed material (matrix). This matrix is generally packed in a column.
Crude cell-lysate samples are loaded onto the column under conditions that ensure the maximum specific binding of the tagged protein to the immobilized ligand. Other proteins that do not contain the "tag" do not bind the immobilized ligand and are washed through. Elution of the bound protein of interest can be achieved by changing the experimental conditions to favour desorption.
ChroMatrix™ affinity chromatography products are based on a superior 6% cross-linked agarose support that ensures fast-flow rates and maximium binding capacity of GST and His-tagged recombinant proteins. Please click on the links below to find out more about a particular product.
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 6% CL-Glutathione ChroMatrix™ - For the purification of GST fusion proteins (AC-201)
6% CL-Nickel ChroMatrix™ - For the purification of His-tagged proteins (AC-202)
6% CL-Cobalt ChroMatrix™ - For the purification of His-tagged proteins (AC-203)
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