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LEXSY FAQs:
Frequently asked questions concerning practical issues when working with LEXSY.
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 Download LEXSY Cloning and Expression FAQ PDF |
LEXSY Grower's FAQs:
Frequently asked questions concerning how to grow a Leishmania culture.
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Download LEXSY Grower's FAQ PDF |
References:
Soleimani M, Mahboudi F, Davoudi N, Amanzadeh A, Azizi M, Adeli A, Rastegar H, Barkhordari F, Mohajer-Maghari B (2007) Expression of human tissue-type plasminogen activator (t-PA) in Leishmania tarentolae. Biotechnology & Applied Biochemistry 48 55-61
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Fritsche C, Sitz M, Weiland N, Breitling R and Pohl HD (2007)Characterization of growth behaviour of Leishmania tarentolae – a new expression system for recombinant proteins. Journal of Basic Microbiology 47: 384-393
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Mureev S, Kushnir S, Kolesnikov AA, Breitling R, Alexandrov K (2007) Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers. Molecular & Biochemical Parasitology 155: 71-83
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Niculae A, Bayer P, Cirstea I, Bergbrede T, Pietrucha R, Gruen M, Breitling R, Alexandrov K (2006) Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae. Protein Expression and Purification 48: 167-172
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Lukeš J, Paris Z, Regmi S, Breitling R, Mureev S, Kushnir S, Pyatkov K, Jirku M and Alexandrov K (2006) Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context. Molecular & Biochemical Parasitology 148: 125-132
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Kushnir S, Gase K, Breitling R and Alexandrov K (2005) Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae. Protein Expression and Purification 42: 37-46
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Lang T, Goyard S, Lebastard M, Milon G (2005) Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cellular Microbiology 7: 383–392
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Barak E, Amin-Spector S, Gerliak E, Goyard S, Holland N and Zilberstein D (2005) Differentiation of Leishmania donovani in host-free system: analysis of signal perception and response.
Molecular & Biochemical Parasitology 141: 99-108
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Sodoyer (2004) Expression Systems for the Production of Recombinant Pharmaceuticals. Biodrugs 18: 51-62
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Breitling R, Klingner S, Callewaert N, Pietrucha R, Geyer A, Ehrlich G, Hartung R, Müller A, Contreras R, Beverley S and Alexandrov K (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expression and Purification 25: 209-218
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