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Fluorescent Labeling by PCR | Fluorescent Labeling by Nick Translation | Biotin/Digoxigenin Labeling by PCR
Fluorescent Labeling by Nick Translation
More Fluorescent Nucleotides for a wide range of applications can be found here!
| Emission Color | Product (Labeling Kit / Dye-Nucleotide) | Absorption max. [nm] | Emission max. [nm] | Common Filter Set | Alternative Fluorophore |
| blue | Atto425 NT Labeling Kit Atto425-dUTP-NT | 436 | 484 | DEAC | DEAC: 430 / 477 nm |
| green | Atto488 NT Labeling Kit Atto488-dUTP-NT | 501 | 523 | Fluorescein | Alexa Fluor 488: 495 / 519 nm Fluorescein (FITC): 495 / 520 nm FAM: 495 / 520 nm Oregon Green 514: 506 / 526 nm Rhodamine green: 503 / 528 nm Rhodamine 123: 507 / 529 nm |
| yellow | Atto550 NT Labeling Kit Atto550-dUTP-NT | 554 | 576 | Cy 3 Spectrum Orange | Alexa Fluor 555: 555 / 565 nm Cy 3: 550 / 570 nm Alexa Fluor 546: 556 / 573 nm TAMRA: 546 / 576 nm Rhodamine Red: 560 / 580 nm Spectrum Orange: 559 / 588 nm |
| orange | Atto590 NT Labeling Kit Atto590-dUTP-NT | 594 | 624 | Texas Red | Texas Red: 583 / 603 nm Alexa Fluor 594: 590 / 617 nm Alexa Fluor 610: 612 / 628 nm |
| red | Atto647N NT Labeling Kit Atto647N-dUTP-NT | 644 | 669 | 669 | Alexa Fluor 647: 650 / 665 nm Cy 5: 643 / 667 nm |
Visible spectrum of light
