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Fluorescent Labeling by PCR  |  Fluorescent Labeling by Nick Translation  |  Non-fluorescent Labeling by PCR

Fluorescent Labeling of DNA by Nick Translation


Emission ColorProduct
(Labeling Kit / Dye-Nucleotide)
Absorption max. [nm]Emission max. [nm]Common Filter SetAlternative Fluorophore
blueAtto425 NT Labeling Kit

Atto425-dUTP-NT
436484DEACDEAC: 430 / 477 nm
greenAtto488 NT Labeling Kit

Atto488-dUTP-NT
501523FluoresceinAlexa Fluor 488: 495 / 519 nm
Fluorescein (FITC): 495 / 520 nm
FAM: 495 / 520 nm
Oregon Green 514: 506 / 526 nm
Rhodamine green: 503 / 528 nm
Rhodamine 123: 507 / 529 nm
yellowAtto550 NT Labeling Kit

Atto550-dUTP-NT
554576Cy 3
Spectrum Orange
Alexa Fluor 555: 555 / 565 nm
Cy 3: 550 / 570 nm
Alexa Fluor 546: 556 / 573 nm
TAMRA: 546 / 576 nm
Rhodamine Red: 560 / 580 nm
Spectrum Orange: 559 / 588 nm
orangeAtto590 NT Labeling Kit

Atto590-dUTP-NT
594624Texas RedTexas Red: 583 / 603 nm
Alexa Fluor 594: 590 / 617 nm
Alexa Fluor 610: 612 / 628 nm
redAtto647N NT Labeling Kit

Atto647N-dUTP-NT
644669669Alexa Fluor 647: 650 / 665 nm
Cy 5: 643 / 667 nm

Visible spectrum of light

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