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Sla I
Isoschizomers: BssH I, PaeR7 I, Sfr274 I, Tli I, Xho I | C↓TCGAG | |
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Cat. No. |
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Price (EUR) |
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S pack |
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EN-134S |
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5000 Units |
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25,00 |
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L pack |
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EN-134L |
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25000 Units |
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100,00 |
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| | Source: Streptomyces lavendulae.
Buffer supplied: 10x B4 (incl. 10x BSA).
Substrate for unit definition: λ DNA, Hind III digest (1 site).
Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Sla I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes. | | |
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