|
|
 |
|
| |
Asu II (isoschizomer)
Isoschizomers: Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, NspV I, Sfu I | TT↓CGAA | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
EN-102S |
|
|
|
3500 Units |
|
|
|
25,00 |
|
|
|
 |
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
EN-102L |
|
|
|
17500 Units |
|
|
|
100,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Source: Actinobacillus suis.
Buffer supplied: 10x B2* and 10x BSA.
Substrate for unit definition: λ DNA, Hind III digest (7 sites).
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.1 % Triton X-100, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15 % Triton X-100, 200 µg /ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Asu II, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes. | | |
Note: BSA is not included into the supplied reaction buffers and should be added separately from the supplied stock solution
(1 mg/ml).
* Buffer contains Triton X-100 to ensure optimal enzyme activity.
Please contact enzymes@jenabioscience.com for more information.
|
|
|
|
