 | Source: Klebsiella pneumoniae OK8.
Buffer supplied: 10x Kpn I and 10x BSA.
Substrate for unit definition: λ DNA, EcoR I digest (2 sites).
Reaction conditions: 10 mM Tris-HCl (pH 7.0 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.01 % Triton X-100, 100 µg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with Kpn I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.
Heat inactivation: No. | |
