 | Source: Bacillus stearothermophilus.
Buffer supplied: 10x BsiS I and 10x BSA.
Substrate for unit definition: λ DNA (328 sites).
Reaction conditions: 66 mM potasium acetate, 33 mM Tris-acetate (pH 7.9 @ 25°C), 10 mM magnesium acetate, 0.5 mM dithiothreitol, 0.1 % Triton X-100, 100 µg/ml BSA. Incubate at 55°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with BsiS I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: No.
Note: Blocked by overlapping dcm methylation. | |
