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		Products & Ordering Nucleosides, Nucleotides and their Analogs Macromolecular Crystallography Eukaryotic Expression System LEXSY PCR-related Products Real-Time PCR Standard PCR RT-PCR Primers and Oligonucleotides DNA Ladders / Protein MW Markers DNA Labeling and Modification DNA Mutagenesis DNA Preparation and Cleanup DNA Sequencing (Sanger Method) Contamination Control Antibodies Recombinant Proteins Affinity Chromatography Enzymes Probes Your Basket Total Products: 154 Total Items: 157 Total Basket Price 35.826,00 EUR View Basket Search & Order Login   Standard PCR - Core Kits  View Basket  Ready-to-Use Mixes / direct gel loading   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   Lyophilisates   |   dNTP Mixes   |   dNTP Bundles   |   Supplements   Complete sets of reagents required for PCR Core Kits provide you premium quality enzymes, ultrapure dNTPs and optimized reaction buffers. Each kit contains complete reaction buffer with MgCl2 ensuring superior results in a broad range of reaction conditions or template-primer conditions. The additional reaction buffer without MgCl2 in combination with the MgCl2 stock solution allows the optimization of magnesium-sensitive PCR reactions. The kit combines simple handling with high flexibility. Standard PCR - Product Selection Guide  Taq Core Kit  Kit of thermostable DNA polymerase, dNTPs and reaction buffer   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-232S 200 units 48,00   L pack PCR-232L 1000 units 192,00 Store at -20°C, avoid frequent thawing and freezing Taq Pol (5 units/µl) dNTP mix 10x Taq reaction buffer complete 10x Taq reaction buffer without MgCl2 MgCl2 stock solution Taq Core Kit is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The buffer system is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required.  Taq Core Kit / high yield  Kit of thermostable DNA polymerase, dNTPs and high yield buffer   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-231S 200 units 48,00   L pack PCR-231L 1000 units 192,00 Store at -20°C, avoid frequent thawing and freezing Taq Pol (5 units/µl) dNTP mix 10x High yield buffer complete 10x High yield buffer without MgCl2 MgCl2 stock solution Taq Core Kit / high yield is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives best results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer system contains detergent and is not recommended for plate based PCR and automated pipetting.  Hot Start Core Kit  Kit of heat-activatable DNA polymerase for high specificity, dNTPs and reaction buffer   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-233S 200 units 83,00   L pack PCR-233L 1000 units 332,00 Store at -20°C, avoid frequent thawing and freezing Hot Start Pol (5 units/µl) dNTP mix 10x Hot Start buffer complete 10x Hot Start buffer without MgCl2 MgCl2 stock solution Hot Start Core Kit provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. Activation step Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.  High Fidelity Core Kit  Kit of thermostable DNA polymerase for high accuracy, dNTPs and reaction buffer   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-234S 100 units 56,00   L pack PCR-234L 500 units 224,00 Store at -20°C, avoid frequent thawing and freezing High Fidelity Pol (2.5 units/µl) dNTP mix 10x High fidelity buffer High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results. High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates. The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. The enzyme is highly purified and free of bacterial DNA.  Pfu-X Core Kit  Kit of proofreading DNA polymerase for highest accuracy, dNTPs and reaction buffer   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-237S 100 units 68,00   L pack PCR-237L 500 units 272,00