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		Products & Ordering Nucleosides, Nucleotides and their Analogs Macromolecular Crystallography Eukaryotic Expression System LEXSY PCR-related Products Real-Time PCR Standard PCR RT-PCR Primers and Oligonucleotides DNA Ladders / Protein MW Markers DNA Labeling and Modification DNA Mutagenesis DNA Preparation and Cleanup DNA Sequencing (Sanger Method) Contamination Control Antibodies Recombinant Proteins Affinity Chromatography Enzymes Probes Your Basket Total Products: 22 Total Items: 22 Total Basket Price 4.580,00 EUR View Basket Search & Order Login   Standard PCR - Thermophilic Polymerases  View Basket  Ready-to-Use Mixes / direct gel loading   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   Lyophilisates   |   dNTP Mixes   |   dNTP Bundles   |   Supplements   Thermophylic Polymerases for Maximum Flexibility Jena Bioscience offers a broad range of optimized thermophilic polymerases. Make your selection from Taq polymerases for routine PCR applications, hot start polymerases for high specific amplifications or proofreading enzyme blends for high fidelity and long range PCR. All enzymes ensure reliable, high performance results and guarantee maximum success for their particular application. Standard PCR - Product Selection Guide  Taq Pol  Thermostable DNA polymerase   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-202S 200 units 35,00   L pack PCR-202L 1000 units 140,00 Store at -20°C, avoid frequent thawing and freezing Taq Pol (5 units/µl) 10x Taq reaction buffer complete 10x Taq reaction buffer without MgCl2 MgCl2 stock solution Taq Pol is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The buffer system is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required. The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.  Taq Pol / high yield  Thermostable DNA polymerase   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-201S 200 units 35,00   L pack PCR-201L 1000 units 140,00 Store at -20°C, avoid frequent thawing and freezing Taq Pol (5 units/µl) 10x High yield buffer complete 10x High yield buffer without MgCl2 MgCl2 stock solution Taq Pol / high yield is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer system contains detergent and is not recommended for plate based PCR and automated pipetting. The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.  Hot Start Pol  Heat-activatable DNA polymerase for high specificity   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-203S 200 units 70,00   L pack PCR-203L 1000 units 280,00 Store at -20°C, avoid frequent thawing and freezing Hot Start Pol (5 units/µl) 10x Hot start buffer complete 10x Hot start buffer without MgCl2 MgCl2 stock solution Hot Start Pol provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity. Activation step Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.  High Fidelity Pol  Thermostable DNA polymerase for high accuracy   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-204S 100 units 48,00   L pack PCR-204L 500 units 192,00 Store at -20°C, avoid frequent thawing and freezing High Fidelity Pol (2.5 units/µl) 10x High fidelity buffer High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates. The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. The enzyme is highly purified and free of bacterial DNA.  Pfu-X Polymerase  Proofreading DNA polymerase for highest accuracy   Product Cat. No. Amount Price (EUR) Buy   S pack PCR-207S 100 units 60,00   L pack PCR-207L 500 units 240,00