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GSH/Nickel/Cobalt Materials - Glutathione ChroMatrix™ |
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6% CL-Glutathione ChroMatrix™ is a superior, 6% cross-linked agarose support with covalently coupled glutathione attached via a 12-atom spacer. 6% CL-Glutathione ChroMatrix™ facilitates the purification of some native Glutathione S Transferase (GST) enzymes and recombinant GST-tagged proteins. It can be used in gravity-flow columns, FPLC and small- or large-scale batches. The material can be reused several times without any significant decrease in yield.
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Features:
- High Binding capacity > 20 mg of protein/ml of ChroMatrix™ resin
- Fast flow rate
- Suitable for GST pull down experiments
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SDS-PAGE analysis of the purification of recombinant human biliverdin-IX reductase
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| lane 1: | molecular mass markers (sizes indicated) | | lane 2: | lysate from cells transformed with control vector (pGEX-KG with no insert) | | lane 3: | lysate from cells transformed with pGEX-BVR-A (with isopropyl-1-thio--D-galactopyranoside) | | lane 4: | lysate from cells transformed with pGEX-BVR-A (without isopropyl-1-thio--D-galactopyranoside) | | lane 5: | purified GST-BVR-A (5 µg) | | lane 6: | cleaved BVR-A (5 µg) |
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| 6% CL-Glutathione ChroMatrix™ | | |
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Product |
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Cat. No. |
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Amount |
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Price (EUR) |
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Buy |
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Bulk material - 10 ml |
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AC-201-10 |
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10 ml |
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140,00 |
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Bulk material - 50 ml |
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AC-201-50 |
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50 ml |
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650,00 |
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Bulk material - 100 ml |
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AC-201-100 |
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100 ml |
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1.300,00 |
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Bulk material - 500 ml |
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AC-201-500 |
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500 ml |
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5.000,00 |
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