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DNA Sequencing (Sanger Method) - Sequencing Polymerases |
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Sequencing Pol
Taq Pol mutant for incorporation of ddNTPs | | |
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Amount |
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Price (EUR) |
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S pack |
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PCR-206S |
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200 units |
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70,00 |
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L pack |
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PCR-206L |
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1000 units |
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280,00 |
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| | Store at -20°C, avoid frequent thawing and freezing
Sequencing Pol (5 unis/µl)
10x Sequencing buffer without MgCl2
MgCl2 stock solution | | Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium and has a 5'→3' polymerisation-dependent exonuclease replacement activity only. | | |
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