|
|
 |
|
|
|
|
|
|
 |
Real-Time PCR - qPCR Mixes with EvaGreen |
|
|
|
|
|
|
|
| |  | |
qPCR Mixes with EvaGreen combine highest convenience with
superior amplification results
The JBS qPCR GreenMaster Series is designed for the quantitative real-time analysis of DNA samples using the fluorescent DNA stain EvaGreen. The fluorescent dye in the master mix intercalates into the amplification product during the PCR process and enables the rapid analysis of target DNA without the need to synthesize sequence-specific labeled probes. They provide easy-to-handle and powerful tools for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision.
The Master contains all reagents required for qPCR (except template and primer) in a premixed 5x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
EvaGreen Fluorescent DNA Stain
EvaGreen Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR), high-resolution DNA melting curve analysis (HRM), routine DNA quantification and capillary gel electrophoresis. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing maximum convenience during routine handling. The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore for real-time PCR applications and a superior replacement for competitor dyes.
|
|
|
|
| |
| qPCR GreenMaster with UNG / ROX | | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
PCR-304S |
|
|
|
100 reactions |
|
|
|
130,00 |
|
|
|
 |
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
PCR-304L |
|
|
|
500 reactions |
|
|
|
520,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Store at 2 to 8°C, if stored at -20°C avoid frequent thawing and freezing
qPCR GreenMaster with UNG / ROX must be stored in the dark
5x qPCR GreenMaster with UNG / ROX
PCR-grade water | | qPCR GreenMaster with UNG / ROX contains UNG (Uracil-N-
Glycosylase) and ROX passive reference dye.
UNG and dUTP instead of dTTP is used to eliminate carry-over contaminations of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | | | |
| |
| qPCR GreenMaster with UNG | | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
PCR-303S |
|
|
|
100 reactions |
|
|
|
130,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
PCR-303L |
|
|
|
500 reactions |
|
|
|
520,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Store at 2 to 8°C, if stored at -20°C avoid frequent thawing and freezing
qPCR GreenMaster with UNG must be stored in the dark
5x qPCR GreenMaster with UNG
PCR-grade water | | qPCR GreenMaster with UNG contains UNG (Uracil-N-Glycosylase).
UNG and dUTP instead of dTTP is used to eliminate carry-over contaminations of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The reaction chemistry of the kit is optimized for block-based PCR instruments. | | | |
| |
| qPCR GreenMaster with ROX | | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
PCR-314S |
|
|
|
100 reactions |
|
|
|
110,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
PCR-314L |
|
|
|
500 reactions |
|
|
|
440,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Store at 2 to 8°C, if stored at -20°C avoid frequent thawing and freezing
qPCR GreenMaster with ROX must be stored in the dark
5x qPCR GreenMaster with ROX
PCR-grade water | | qPCR GreenMaster with ROX contains ROX passive reference dye.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal. | | | |
| |
| qPCR GreenMaster | | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
PCR-313S |
|
|
|
100 reactions |
|
|
|
110,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
PCR-313L |
|
|
|
500 reactions |
|
|
|
440,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Store at 2 to 8°C, if stored at -20°C avoid frequent thawing and freezing
qPCR GreenMaster must be stored in the dark
5x qPCR GreenMaster
PCR-grade water | | qPCR GreenMaster is optimized for block-based PCR instruments. | | |
EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
|
|
|
|
