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DNA Manipulation - Inactivation of Restriction Enzyme Recognition Sites |
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5-Methyl-dCTP, S pack |
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NU-1125S |
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100 Units |
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98,50 |
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5-Methyl-dCTP, L pack |
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NU-1125L |
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500 Units |
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314,50 |
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5-AcOHg-dCTP, S pack |
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NU-1149S |
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100 Units |
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98,50 |
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5-AcOHg-dCTP, L pack |
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NU-1149L |
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500 Units |
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314,50 |
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Description
5-Methyl-dCTP is widely used for construction of cDNA libraries
| Incorporation of 5-Methyl-dCTP
M-MuLV Reverse Transcriptase
Klenow Fragment of DNA Polymerase I
Sequenase DNA Polymerase
(Taq Polymerase, Vent) *
Incorporation of Hg-dCTP
DNA Polymerase I
References to 5-Methyl-dCTP
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584.
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 (3):681.
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research 6:369.
* Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 (5):1081.
Reference to Hg-dCTP
Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445.
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