Products & Ordering   Order Information   About us   Contact   Downloads   Blog  Search
		Products & Ordering Nucleosides, Nucleotides and their Analogs Macromolecular Crystallography Eukaryotic Expression System LEXSY PCR-related Products Real-Time PCR Standard PCR RT-PCR Primers and Oligonucleotides DNA Ladders / Protein MW Markers DNA Labeling and Modification DNA Mutagenesis DNA Preparation and Cleanup DNA Sequencing (Sanger Method) Contamination Control Antibodies Recombinant Proteins Affinity Chromatography Enzymes Probes Your Basket Total Products: 64 Total Items: 64 Total Basket Price 16.799,00 EUR View Basket Search & Order Login   DNA Mutagenesis - Random Mutagenesis Kits  View Basket  Random Mutagenesis Kits Within three billion years of evolution, nature has produced a plethora of proteins simply by repeated cycles of random mutagenesis followed by in vivo selection for superior function of the encoded proteins. This example of natural evolution has guided researchers within the last two decades to develop strategies for in vitro permutation of proteins. Among the variety of strategies applied, three major powerful techniques have emerged.  JBS dNTP-Mutagenesis Kit  Random Mutagenesis by dNTP Analogs   Product Cat. No. Amount Price (EUR) Buy   Kit PP-101 15 reactions 240,00   Store at -20°C, avoid frequent thawing and freezing Polymerase 10x Mutagenesis buffer dNTP mix dPTP 8-oxo-dGTP PCR grade water JBS dNTP-Mutagenesis Kit is based on the incorporation of the mutagenic dNTP analogs 8-oxo-dGTP and dPTP into an amplified DNA fragment by PCR. The mutagenic dNTPs are eliminated by a second PCR step in the presence of the four natural dNTPs only, resulting in a rate of mutagenesis of up to 20%.  JBS Error-Prone Kit  Random Mutagenesis by Error-Prone PCR   Product Cat. No. Amount Price (EUR) Buy   Kit PP-102 15 reactions 190,00   Store at -20°C, avoid frequent thawing and freezing Polymerase 10x Reaction buffer 10x Error-prone solution dNTP error-prone mix PCR grade water JBS Error-Prone Kit introduces mutations in the gene of interest using modified PCR reaction conditions. An increased MgCl2 concentration, the addition of MnCl2 and the unbalanced ratio of dNTPs induce an increased error-rate of the DNA-polymerase. The rate of mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0%.  JBS DNA-Shuffling Kit  Random Mutagenesis by DNA Shuffling   Product Cat. No. Amount Price (EUR) Buy   Kit PP-103 15 reactions 240,00   Store at -20°C, avoid frequent thawing and freezing DNase I 10x Digestion buffer DNase stop solution Polymerase 10x Shuffling buffer dNTP mix PCR grade water JBS DNA-Shuffling Kit is designed to generate gene libraries by random fragmentation of one gene or a pool of related genes. A controlled fragmentation of the DNA is followed by a random reassembly in a self-priming PCR reaction. This method allows the recombination of sequences from different, related genes. The overall rate of mutagenesis is approx. 0.7%. For inquiries or further information, please contact us at pcr@jenabioscience.com

      © 2009 Jena Bioscience GmbH - Imprint - Design and CMS by timespin Digital Communication GmbH