Jena Bioscience offers versatile fluorescent labeling kits for either amine or thiol labeling of proteins.
In general, thiol labeling is more specific than amine labeling due to the relatively low abundance of cysteines compared to lysines. Since however, appearance of cysteines and lysines varies among different proteins, the amino acid sequence and tertiary structure of the protein of interest must be analyzed prior to deciding on the labeling strategy.
The intended application of the fluorescent protein also determines the labeling strategy. Amine labeling is used for immunochemistry whereas thiol labeling is preferred for investigating structure, function and protein interaction.
The outstanding photostability and quantum yield of the thoroughly selected Atto-dyes combined with a straightforward labeling protocol provide high-quality kits for fluorescent labeling of proteins.