DNA probes up to 4 kbp: Efficient Cy®-, ATTO™- or Alexa Fluor®-dye labeling

Labeled DNA probes e.g. for (Fluorescence) in situ hybridization ((F)ISH) are routinely prepared via PCR if template amounts are limited or labeling of sequence-specific DNA fragment is required.

More information...

We now offer HighFidelity PCR Labeling Kits...

...for both fluorescent (e.g Cy3 or Alexa Fluor 488), Biotin and Digoxigenin labeling
...suitable for preparation of both short (>100 bp) and long DNA probes (up to 4 kbp tested) due to a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme (Fig. 1).
...with convenient configurations for application-specific assay optimization:
- single nucleotide format for optimization of labeled dUTP/dTTP ratio
- positive control reaction (amplification of 500 bp fragment)
- testkits containing selected labeled dUTPs to find the optimal label

Figure 1: High Fidelity Polymerase allows efficient production of 4 kbp labeled DNA probes.

Taq polymerase- and High Fidelity Polymerase-mediated production of 4 kbp labeled DNA probes has been compared in the presence of varying amounts of labeled dUTP. Visualization of analog incorporation was performed by agarose gel electrophoresis without DNA intercalator. Product formation was visualized by agarose gel electrophoresis with DNA intercalator EtBr. A) Production of 4 kbp ATTO488-labeled DNA probe with dUTP-XX-ATTO-488. B) Production of 4 kbp Digoxigenin-labeled DNA probe with DIG-11-dUTP. Band shift indicates Dig-11-dUTP incorporation. M: DNA Ladder (Cat. No. M-216), 0 - 100 %: Increasing amount of dTTP substitution by labeled dUTP.

Table 1: Overview on available HighFidelity PCR Labeling Kits (* AF: Alexa Fluor)

Kit	Introduced Label	Underlying labeled nucleotide
Hapten HighFidelity PCR Labeling Kits
HighFidelity Digoxigenin PCR Labeling Kit	Digoxigenin	Digoxigenin-11-dUTP
HighFidelity Biotin16 PCR Labeling Kit	Biotin	Biotin-16-dUTP
Fluorescent HighFidelity PCR Labeling Kits
HighFidelity Fluorescein PCR Labeling Kit	Fluorescein	Fluorescein-12-dUTP
HighFidelity ATTO488 PCR Labeling Kit	ATTO488	dUTP-XX-ATTO-488
HighFidelity AF*488 PCR Labeling Kit	AF*488	dUTP-XX-AF*488
HighFidelity GREEN PCR Labeling Testkit	Fluorescein ATTO488 AF*488	Fluorescein-12-dUTP dUTP-XX-ATTO-488 dUTP-XX-AF*488
HighFidelity Cy3 PCR Labeling Kit	Cy3	dUTP-XX-Cy3
HighFidelity AF*555 PCR Labeling Kit	AF*555	dUTP-XX-AF*555
HighFidelity YELLOW PCR Labeling Testkit	Cy3 AF*555	dUTP-Cy3 dUTP-XX-Cy3 dUTP-XX-AF*555
HighFidelity AF*594 PCR Labeling Kit	AF*594	dUTP-XX-AF*594
HighFidelity ATTO594 PCR Labeling Kit	ATTO594	dUTP-XX-ATTO-594
HighFidelity ORANGE PCR Labeling Testkit	TexasRed AF*594 ATTO594	dUTP-TexasRed dUTP-XX-AF*594 dUTP-XX-ATTO-594
HighFidelity Cy5 PCR Labeling Kit	Cy5	dUTP-Cy5
HighFidelity AF*647 PCR Labeling Kit	AF*647	dUTP-XX-AF*647
HighFidelity ATTO643 PCR Labeling Kit	ATTO643	dUTP-ATTO-643
HighFidelity RED PCR Labeling Testkit	Cy5 AF*647 ATTO643	dUTP-Cy5 dUTP-XX-AF*647 dUTP-ATTO-643

Properties of Fluorescent Dyes, Fluorescent Quencher & Redox Label

Nick Translation Labeling Kits for fluorescent or Hapten Labeling are available as well.

Didn’t find the labeling kit you are looking for? Please contact me at
info@jenabioscience.com!

DNA probes up to 4 kbp: Efficient Cy®-, ATTO™- or Alexa Fluor®-dye labeling

We now offer HighFidelity PCR Labeling Kits...

Table 1: Overview on available HighFidelity PCR Labeling Kits (* AF: Alexa Fluor)

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